Chemical Derivatization of Affinity Matrices Provides Protection from Tryptic Proteolysis.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00254
Abstract: The enrichment of biotinylated proteins using immobilized streptavidin has become a staple methodology for affinity purification-based proteomics. Many of these workflows rely upon tryptic digestion to elute streptavidin-captured moieties from the beads. The concurrent release… read more here.
Keywords: affinity matrices; proteolysis; affinity; streptavidin ... See more keywords
Rational design and synthesis of affinity matrices based on proteases immobilized onto cellulose membranes
Sign Up to like & getrecommendations! Published in 2017 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2017.1315600
Abstract: ABSTRACT Discovery of new protease inhibitors may result in potential therapeutic agents or useful biotechnological tools. Obtainment of these molecules from natural sources requires simple, economic, and highly efficient purification protocols. The aim of this… read more here.
Keywords: affinity matrices; immobilization; affinity; rational design ... See more keywords