An epitope‐directed antibody affinity maturation system utilizing mammalian cell survival as readout
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26965
Abstract: Upon developing therapeutically potent antibodies, there are significant requirements, such as increasing their affinity, regulating their epitope, and using native target antigens. Many antibody selection systems, such as a phage display method, have been developed,… read more here.
Keywords: cell; system; affinity; antibody affinity ... See more keywords
In vitro affinity maturation to improve the efficacy of a hypoxia-inducible factor 1α single-domain intrabody.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.06.097
Abstract: Affinity is an important property of therapeutic antibodies, so improving affinity is critical to the biological activity and clinical efficacy. An anti-HIF-1α nanobody, VHH212, was screened via a native ribosome display library with a 26.6 nM… read more here.
Keywords: intrabody; vitro affinity; maturation improve; affinity ... See more keywords
Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease
Sign Up to like & getrecommendations! Published in 2019 at "Cell reports"
DOI: 10.1016/j.celrep.2019.06.066
Abstract: SUMMARY Pemphigus vulgaris (PV) is an autoimmune disease characterized by blistering sores on skin and mucosal membranes, caused by autoantibodies primarily targeting the cellular adhesion protein, desmoglein-3 (Dsg3). To better understand how Dsg3-specific autoantibodies develop… read more here.
Keywords: pemphigus vulgaris; vulgaris autoimmune; disease; autoimmune disease ... See more keywords
Affinity maturation in a human humoral response to influenza hemagglutinin
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1915620116
Abstract: Significance Influenza virus and some other human pathogens evolve to evade herd immunity and, hence, to reinfect previously immune individuals. Individual humoral immune responses also evolve, through somatic mutation and selection in a process known… read more here.
Keywords: influenza hemagglutinin; affinity maturation; response; affinity ... See more keywords
Fast-tracking antibody maturation using a B cell-based display system
Sign Up to like & getrecommendations! Published in 2022 at "mAbs"
DOI: 10.1080/19420862.2022.2122275
Abstract: ABSTRACT Affinity maturation, an essential component of antibody engineering, is crucial for developing therapeutic antibodies. Cell display system coupled with somatic hypermutation (SHM) initiated by activation-induced cytidine deaminase (AID) is a commonly used technique for… read more here.
Keywords: system; cell; maturation; affinity maturation ... See more keywords
SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz050
Abstract: Abstract Mutagenesis libraries are essential for combinatorial protein engineering. Despite improvements in gene synthesis and directed mutagenesis, current methodologies still have limitations regarding the synthesis of complete antibody single-chain variable fragment (scFv) genes and simultaneous… read more here.
Keywords: mutagenesis; antibody affinity; affinity maturation; solid phase ... See more keywords
Proteogenomic analysis of the autoreactive B cell repertoire in blood and tissues of patients with Sjögren’s syndrome
Sign Up to like & getrecommendations! Published in 2022 at "Annals of the Rheumatic Diseases"
DOI: 10.1136/annrheumdis-2021-221604
Abstract: Objective To comparatively analyse the aberrant affinity maturation of the antinuclear and rheumatoid factor (RF) B cell repertoires in blood and tissues of patients with Sjögren’s syndrome (SjS) using an integrated omics workflow. Methods Peptide… read more here.
Keywords: blood; repertoire; affinity maturation; cell ... See more keywords
Rapid Affinity Maturation of Novel Anti-PD-L1 Antibodies by a Fast Drop of the Antigen Concentration and FACS Selection of Yeast Libraries
Sign Up to like & getrecommendations! Published in 2019 at "BioMed Research International"
DOI: 10.1155/2019/6051870
Abstract: The affinity engineering is a key step to increase the efficacy of therapeutic monoclonal antibodies and yeast surface display is the most widely used and powerful affinity maturation approach, achieving picomolar binding affinities. In this… read more here.
Keywords: affinity; selection; yeast; yeast libraries ... See more keywords
Repertoire Analysis of Antibody CDR-H3 Loops Suggests Affinity Maturation Does Not Typically Result in Rigidification
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2018.00413
Abstract: Antibodies can rapidly evolve in specific response to antigens. Affinity maturation drives this evolution through cycles of mutation and selection leading to enhanced antibody specificity and affinity. Elucidating the biophysical mechanisms that underlie affinity maturation… read more here.
Keywords: affinity; analysis; antibody; flexibility ... See more keywords
Local and Global Rigidification Upon Antibody Affinity Maturation
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2020.00182
Abstract: During the affinity maturation process the immune system produces antibodies with higher specificity and activity through various rounds of somatic hypermutations in response to an antigen. Elucidating the affinity maturation process is fundamental in understanding… read more here.
Keywords: local global; maturation; global rigidification; affinity maturation ... See more keywords
Enhancers Improve the AID-Induced Hypermutation in Episomal Vector for Antibody Affinity Maturation in Mammalian Cell Display
Sign Up to like & getrecommendations! Published in 2018 at "Antibodies"
DOI: 10.3390/antib7040042
Abstract: The induction of somatic hypermutation (SHM) in various cell lines by activation-induced cytidine deaminase (AID) has been used in protein-directed selection, especially in antibody affinity maturation. Several antibody affinity maturation systems based on mammalian cells… read more here.
Keywords: antibody; antibody affinity; aid; hypermutation ... See more keywords