The future of protein scaffolds as affinity reagents for purification
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26090
Abstract: Affinity purification is one of the most powerful separation techniques extensively employed both at laboratory and production scales. While antibodies still represent the gold standard affinity reagents, others derived from non‐immunoglobulin scaffolds emerged as interesting… read more here.
Keywords: immunoglobulin scaffolds; affinity; affinity purification; affinity reagents ... See more keywords
Analysis of FGF20‐regulated genes in organ of Corti progenitors by translating ribosome affinity purification
Sign Up to like & getrecommendations! Published in 2020 at "Developmental Dynamics"
DOI: 10.1002/dvdy.211
Abstract: Understanding the mechanisms that regulate hair cell (HC) differentiation in the organ of Corti (OC) is essential to designing genetic therapies for hearing loss due to HC loss or damage. We have previously identified Fibroblast… read more here.
Keywords: ribosome affinity; fgf20; organ corti; affinity purification ... See more keywords
DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1217-0_6
Abstract: The interaction of proteins with DNA plays a central role in gene regulation. We describe a DNA affinity purification method that allows for identification and analysis of protein complex components. For example, a DNA probe… read more here.
Keywords: dna affinity; affinity purification; proteins binding; purification pulldown ... See more keywords
Using Tandem Affinity Purification to Identify Circadian Clock Protein Complexes from Arabidopsis.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1912-4_15
Abstract: Identification of protein-protein interactions is an effective method of elucidating new roles for circadian clock-associated proteins that can expand beyond the information collected from transcriptional studies and genetic screens. Tandem affinity purification coupled with liquid… read more here.
Keywords: protein; tandem affinity; circadian clock; affinity purification ... See more keywords
Affinity Purification Followed by Liquid Chromatography-Tandem Mass Spectrometry to Identify Proteins Interacting with ABA Signaling Components.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2156-1_14
Abstract: Abscisic acid (ABA) is a key phytohormone involved in plant development, seed germination and responses to osmotic stresses, such as drought and high salinity. SNF1-related protein kinases (SnRK2s) play important roles in ABA-dependent and ABA-independent… read more here.
Keywords: stress; stress signaling; aba osmotic; affinity purification ... See more keywords
Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6518-2_2
Abstract: Protein purification by tandem affinity purification (TAP)-tag coupled to mass spectrometry analysis is usually used to reveal protein complex composition. Here we describe a TAP-tag purification of chromatin-bound proteins along with associated nucleosomes, which allow… read more here.
Keywords: tandem affinity; mass spectrometry; chromatin; affinity purification ... See more keywords
Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7537-2_13
Abstract: Peptidomic techniques are powerful tools to identify peptides in a biological sample. This protocol describes a targeted peptidomic approach that uses affinity chromatography to purify peptides that are substrates of carboxypeptidase E (CPE), an enzyme… read more here.
Keywords: affinity; cpe substrates; purification neuropeptide; affinity purification ... See more keywords
Characterization of a functional recombinant human creatine kinase-MB isoenzyme prepared by tandem affinity purification from Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8286-5
Abstract: Creatine kinase isoform CK-MB has been widely applied as a biomarker of myocardial injury. While a variety of methods have been used to measure CK-MB activity or mass in clinical laboratories, a CK-MB standard is… read more here.
Keywords: creatine; tandem affinity; human creatine; affinity purification ... See more keywords
Novel interacting proteins identified by tandem affinity purification coupled to nano LC-MS/MS interact with ribosomal S6 protein kinase 4 (RSK4) and its variant protein (RSK4m).
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2016.12.030
Abstract: Ribosomal S6 protein kinase 4 (RSK4) is an important novel tumor suppressor that inhibits breast cancer cell growth and induces senescence. However, RSK4m, which is a variant of RSK4 resulting from alternative splicing, may play… read more here.
Keywords: protein; tandem affinity; rsk4 rsk4m; rsk4 ... See more keywords
Using Affinity Purification Coupled with Stable Isotope Labeling by Amino Acids in Cell Culture Quantitative Mass Spectrometry to Identify Novel Interactors/Substrates of Protein Arginine Methyltransferases.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.11.015
Abstract: The protein arginine methyltransferase family (PRMT) is known as being the catalytic driving force for arginine methylation. This specific type of post translational modification is extensively used in biological processes, and therefore is highly relevant… read more here.
Keywords: interactors substrates; identify novel; purification coupled; protein arginine ... See more keywords
Developing a Bimolecular Affinity Purification Strategy to Isolate 26S Proteasome Holocomplexes for Complex-Centric Proteomic Analysis.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03551
Abstract: The 26S proteasome is a mega-dalton protein complex responsible for intracellular degradation in eukaryotes. It is composed of two subcomplexes: the 20S core particle and the 19S regulatory particle, which form compositionally and structurally heterogeneous… read more here.
Keywords: analysis; 26s proteasome; affinity purification; strategy ... See more keywords