The future of protein scaffolds as affinity reagents for purification
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26090
Abstract: Affinity purification is one of the most powerful separation techniques extensively employed both at laboratory and production scales. While antibodies still represent the gold standard affinity reagents, others derived from nonâimmunoglobulin scaffolds emerged as interesting… read more here.
Keywords: immunoglobulin scaffolds; affinity; affinity purification; affinity reagents ... See more keywords
Generation of recombinant affinity reagents against a two-phosphosite epitope of ATF2.
Sign Up to like & getrecommendations! Published in 2018 at "New biotechnology"
DOI: 10.1016/j.nbt.2017.10.004
Abstract: Activating Transcription Factor 2 (ATF2) plays an important role in mammalian cell proliferation, apoptosis and DNA repair. Its activation is dependent on the sequential phosphorylation of residue threonine 71 (T71) followed by threonine 69 (T69)… read more here.
Keywords: recombinant affinity; affinity reagents; atf2; epitope ... See more keywords
A phage display-based strategy for the de novo creation of disulfide-constrained and isomer-free bicyclic peptide affinity reagents.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical communications"
DOI: 10.1039/c7cc09142g
Abstract: Bicyclic peptides have been attractive scaffolds for developing high affinity reagents for biomacromolecules. Here we report a general phage-screening strategy for the development of bicyclic peptide ligands constrained with isomerically-forbidden disulfide bridges without elaborate chemical… read more here.
Keywords: strategy; bicyclic peptide; affinity reagents; disulfide ... See more keywords