RNA-ALIS: Methodology for screening soluble RNAs as small molecule targets using ALIS affinity-selection mass spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.04.024
Abstract: Recent advances resulting from the completion of the human genome have shown that RNA has the promise to be a target for small molecule drugs, and therefore represents a previously unexploited class of targets for… read more here.
Keywords: affinity selection; methodology; small molecule; selection mass ... See more keywords
Advances in Magnetic Microbead Affinity Selection Screening: Discovery of Natural Ligands to the SARS-CoV-2 Spike Protein.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.1c00318
Abstract: Affinity selection-mass spectrometry, which includes magnetic microbead affinity selection-screening (MagMASS), is ideal for the discovery of ligands in complex mixtures that bind to pharmacological targets. Therapeutic agents are needed to prevent or treat COVID-19, which… read more here.
Keywords: magnetic microbead; protein; sars cov; affinity selection ... See more keywords
Robustness of In Vitro Selection Assays of DNA-Encoded Peptidomimetic Ligands to CBX7 and CBX8
Sign Up to like & getrecommendations! Published in 2018 at "SLAS Discovery"
DOI: 10.1177/2472555217750871
Abstract: The identification of protein ligands from a DNA-encoded library is commonly conducted by an affinity selection assay. These assays are often not validated for robustness, raising questions about selections that fail to identify ligands and… read more here.
Keywords: affinity selection; dna encoded; selection assays; selection ... See more keywords
Design and Development of a Technology Platform for DNA-Encoded Library Production and Affinity Selection
Sign Up to like & getrecommendations! Published in 2018 at "SLAS Discovery"
DOI: 10.1177/2472555217752091
Abstract: DNA-encoded libraries (DELs) have emerged as an efficient and cost-effective drug discovery tool for the exploration and screening of very large chemical space using small-molecule collections of unprecedented size. Herein, we report an integrated automation… read more here.
Keywords: affinity selection; production affinity; dna encoded; affinity ... See more keywords
Identification of G-Quadruplex-Binding Inhibitors of Myc Expression through Affinity Selection–Mass Spectrometry
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DOI: 10.1177/2472555218796656
Abstract: The Myc oncogene is overexpressed in many cancers, yet targeting it for cancer therapy has remained elusive. One strategy for inhibition of Myc expression is through stabilization of the G-quadruplex (G4), a G-rich DNA secondary… read more here.
Keywords: affinity selection; selection mass; mass spectrometry; myc expression ... See more keywords