A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7183-1_25
Abstract: Sterols are major constituents of the plasma membrane of eukaryotic cells and serve as a precursor for several classes of signaling molecules, including steroids and hydroxy sterols. They maintain the functionality and permeability barrier of… read more here.
Keywords: binding assay; specificity sterol; assay measure; measure affinity ... See more keywords
Modulating binding affinity, specificity and configurations by multivalent interactions.
Sign Up to like & getrecommendations! Published in 2022 at "Biophysical journal"
DOI: 10.1016/j.bpj.2022.04.017
Abstract: Biological functions of proteins rely on their specific interactions with binding partners. Many proteins contain multiple domains, which can bind to their targets that often have more than one binding site, resulting in multivalent interactions.… read more here.
Keywords: domain; binding affinity; multivalent interactions; affinity specificity ... See more keywords
Systematically Modulating Aptamer Affinity and Specificity by Guanosine-to-Inosine Substitution.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00422
Abstract: Aptamers are widely used in small molecule detection applications due to their specificity, stability, and cost effectiveness. One key challenge in utilizing aptamers in sensors is matching the binding affinity of the aptamer to the… read more here.
Keywords: affinity specificity; affinity; specificity guanosine; guanosine inosine ... See more keywords
Multifunctional Screening Platform for the Highly Efficient Discovery of Aptamers with High Affinity and Specificity.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00684
Abstract: Aptamers have attracted much attention as the next generation of affinity reagents. Unfortunately, the selection efficiency remains a critical bottleneck for the widespread application of aptamers. Herein, to accelerate aptamers discovery, a multifunctional microfluidic selection… read more here.
Keywords: high affinity; affinity; selection; platform ... See more keywords
Panel of Engineered Ubiquitin Variants Targeting the Family of Human Ubiquitin Interacting Motifs.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00089
Abstract: Ubiquitin (Ub)-binding domains embedded in intracellular proteins act as readers of the complex Ub code and contribute to regulation of numerous eukaryotic processes. Ub-interacting motifs (UIMs) are short α-helical modular recognition elements whose role in… read more here.
Keywords: variants targeting; panel engineered; interacting motifs; ubiquitin variants ... See more keywords
Affinity, Specificity, and Cooperativity of DNA Binding by Bacterial Gene Regulatory Proteins
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23010562
Abstract: Nearly all of biology depends on interactions between molecules: proteins with small molecules, proteins with other proteins, nucleic acids with small molecules, and nucleic acids with proteins that regulate gene expression, our concern in this… read more here.
Keywords: dna; affinity specificity; gene; cooperativity ... See more keywords