Overexpression and characterization of a novel GH16 β-agarase (Aga1) from Cellulophaga omnivescoria W5C
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Letters"
DOI: 10.1007/s10529-020-02933-x
Abstract: To identify and characterize a new β-agarase from Cellulophaga omnivescoria W5C capable of producing biologically-active neoagarooligosaccharides from agar. The β-agarase, Aga1, has signal peptides on both N- and C-terminals, which are involved in the type… read more here.
Keywords: agarase; agarase aga1; cellulophaga omnivescoria; omnivescoria w5c ... See more keywords
Purification and Characterization of Agarase from Marine Bacteria Acinetobacter sp. PS12B and Its Use for Preparing Bioactive Hydrolysate from Agarophyte Red Seaweed Gracilaria verrucosa
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-018-2726-2
Abstract: Acinetobacter strain PS12B was isolated from marine sediment and was found to be a good candidate to degrade agar and produce agarase enzyme. The extracellular agarase enzyme from strain PS12B was purified by ammonium sulfate… read more here.
Keywords: agarase; gracilaria verrucosa; enzyme; purification ... See more keywords
Characterization of a Novel Neoagarobiose-Producing GH42 β-Agarase, AgaJ10, from Gayadomonas joobiniege G7
Sign Up to like & getrecommendations! Published in 2019 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-019-02992-5
Abstract: Gayadomonas joobiniege G7 is an agar-degrading bacterium, which produces various agarases that have been biochemically characterized recently. In this study, we biochemically characterized a new β-agarase AgaJ10 belonging to the glycoside hydrolase (GH) 42 family… read more here.
Keywords: agarase; gh42 agarase; gayadomonas joobiniege; agarase agaj10 ... See more keywords
Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-018-0072-4
Abstract: The gene coding for a thermotolerant β-agarase from an isolated Simiduia sp. SH-4 was cloned, recombinantly expressed, and characterized after purification. This gene was sequenced after cassette mediated polymerase chain reaction and composed of an… read more here.
Keywords: agarase; expression characterization; agarase simiduia; thermotolerant agarase ... See more keywords
Enhanced a novel β-agarase production in recombinant Escherichia coli BL21 (DE3) through induction mode optimization and glycerol feeding strategy
Sign Up to like & getrecommendations! Published in 2018 at "Acta Oceanologica Sinica"
DOI: 10.1007/s13131-018-1172-x
Abstract: Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food, cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode… read more here.
Keywords: agarase; production; agarase production; induction ... See more keywords
Overexpression and characterization of a thermostable β-agarase producing neoagarotetraose from a marine isolate Microbulbifer sp. AG1
Sign Up to like & getrecommendations! Published in 2019 at "Acta Oceanologica Sinica"
DOI: 10.1007/s13131-019-1349-y
Abstract: An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was… read more here.
Keywords: agarase; microbulbifer ag1; overexpression characterization; characterization thermostable ... See more keywords
Mutagenesis on the surface of a β-agarase from Vibrio sp. ZC-1 increased its thermo-stability.
Sign Up to like & getrecommendations! Published in 2019 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.04.006
Abstract: The recombinant rAgaZC-1 was a family GH50 β-agarase from Vibrio sp. ZC-1 (CICC 24670). In this paper, the mutant D622G (i.e., mutate the aspartic acid at position 622 to glycine) had better thermo-stability than rAgaZC-1,… read more here.
Keywords: agarase; agarase vibrio; ragazc; thermo stability ... See more keywords
Heterologous expression of an agarase gene in Bacillus subtilis, and characterization of the agarase.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.07.118
Abstract: A β-agarase was identified from Pseudoalteromonas sp. Q30F and heterologously expressed in Bacillus subtilis WB800n. The β-agarase, Aga862 encoded by aga862 gene in an open reading frame of 1338 bp is 445 amino acids in length,… read more here.
Keywords: agarase; subtilis wb800n; heterologous expression; gene ... See more keywords
Characterizing of a new α-agarase AgaE from Thalassomonas sp. LD5 and probing its catalytically essential residues.
Sign Up to like & getrecommendations! Published in 2021 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2021.11.194
Abstract: A new α-agarase AgaE belonging to glycoside hydrolase (GH) family 96 was identified and cloned from marine bacterium Thalassomonas sp. LD5. AgaE consists of 926 amino acids with a theoretical molecular mass of 97 kDa. The… read more here.
Keywords: thalassomonas ld5; new agarase; agae; amino acids ... See more keywords
Identification and characterization of a thermostable endolytic β-agarase Aga2 from a newly isolated marine agarolytic bacteria Cellulophaga omnivescoria W5C.
Sign Up to like & getrecommendations! Published in 2018 at "New biotechnology"
DOI: 10.1016/j.nbt.2017.09.006
Abstract: Research on the enzymatic breakdown of seaweed-derived agar has recently gained attention due to the progress in green technologies for marine biomass utilization. The enzymes known as agarases catalyze the cleavage of glycosidic bonds within… read more here.
Keywords: agarase; agarase aga2; omnivescoria w5c; cellulophaga omnivescoria ... See more keywords
Characterization of a GH50 β-Agarase: A Biotechnological Tool for Preparing Oligosaccharides from Agarose and Porphyran.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c02107
Abstract: Agarase is of vital significance for functional agaro-oligosaccharides production from algal dived agarose. Especially, the exolytic agarases have the advantage of obtaining agaro-oligosaccharides with a specific degree of polymerization. Herein, we cloned and expressed a… read more here.
Keywords: ouc pgjc50; characterization gh50; agarase; gh50 agarase ... See more keywords