Co-culturing on dry filter paper significantly increased the efficiency of Agrobacterium-mediated transformations of maize immature embryos
Sign Up to like & getrecommendations! Published in 2018 at "Physiology and Molecular Biology of Plants"
DOI: 10.1007/s12298-018-00641-5
Abstract: In the Agrobacterium tumefaciens-mediated transformations of maize immature embryos (IEs), the common co-culturing media used are MS or N6-based (MC). Here, we used a novel co-culturing method in which maize ‘Qi319’ IEs inoculated with Agrobacterium-harboring… read more here.
Keywords: maize immature; mediated transformations; transformations maize; agrobacterium ... See more keywords
Evaluation of the interspecific competition within Agrobacterium spp. in the soil and rhizosphere of tomato and maize
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Plant Pathology"
DOI: 10.1007/s42161-018-0114-y
Abstract: Agrobacterium biovar 1 is responsible for crown gall disease of many plant species. However, according to the current species definition, this taxon displays too large a genomic divergence to be a single species. This is… read more here.
Keywords: plant; tomato maize; agrobacterium; soil rhizosphere ... See more keywords
Agrobacterium strains and strain improvement: Present and outlook.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2020.107677
Abstract: Almost 40 years ago the first transgenic plant was generated through Agrobacterium tumefaciens-mediated transformation, which, until now, remains the method of choice for gene delivery into plants. Ever since, optimized Agrobacterium strains have been developed with… read more here.
Keywords: transformation; agrobacterium; agrobacterium strains; strain improvement ... See more keywords
Establishment of Agrobacterium tumefaciens - mediated genetic transformation of apple pathogen Marssonina coronaria using marker genes under the control of CaMV 35S promoter.
Sign Up to like & getrecommendations! Published in 2021 at "Microbiological research"
DOI: 10.1016/j.micres.2021.126878
Abstract: Premature leaf fall of apple caused by Marssonina coronaria is economically very important apple disease and all the commercially available apple cultivars are susceptible to this disease. The non-availability of an efficient transformation system for… read more here.
Keywords: camv 35s; transformation; 35s promoter; agrobacterium ... See more keywords
A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.01.015
Abstract: In recent years, versatile genetic tools have been developed and applied to a number of filamentous fungi of industrial importance. However, the existing techniques have limitations when it comes to achieve the desired genetic modifications,… read more here.
Keywords: agrobacterium; gene targeting; aspergillus carbonarius; gene ... See more keywords
Optimization of regeneration and Agrobacterium-mediated transformation of Stevia (Stevia rebaudiana Bertoni): a commercially important natural sweetener plant
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-72751-8
Abstract: Stevia rebaudiana Bertoni is a commercially important zero calorie natural-sweetener herb which produce sweet compounds known as steviol glycosides. Rising demands of steviol glycosides by food and beverage industries has led to an increase in… read more here.
Keywords: stevia; stevia rebaudiana; bertoni commercially; transformation ... See more keywords
Agrobacterium-Mediated Insect Resistance Gene (cry1Aa) Transfer Studies Pertaining to Antibiotic Sensitivity on Cultured Tissues of Broccoli
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Vegetable Science"
DOI: 10.1080/19315260.2017.1334734
Abstract: ABSTRACT Successful plant transformation requires an efficient regeneration protocol and a suitable selection method. A genetically engineered, disarmed Agrobacterium tumefaciens strain containing a binary vector pBin-1Aa with cry1Aa (insect resistance) and npt-II (neomycin phosphotransferase-II) genes… read more here.
Keywords: tissues broccoli; pertaining antibiotic; regeneration; insect resistance ... See more keywords
Isolation and characterization of the 2,5-pyridinedicarboxylic acid-degrading bacterium Agrobacterium sp. strain YJ-5.
Sign Up to like & getrecommendations! Published in 2023 at "Letters in applied microbiology"
DOI: 10.1093/lambio/ovac040
Abstract: 2,5-Pyridinedicarboxylic acid (2,5-PDA), a natural N-heterocyclic compound and a substitute for production in plastics, was widely distributed in industrial wastewater. However, the biodegradation of 2,5-PDA has been rarely reported. In this study, strain YJ-5, which… read more here.
Keywords: isolation characterization; agrobacterium; strain; pyridinedicarboxylic acid ... See more keywords
Isothermal Amplification and Lateral-Flow Assay for Detecting Crown-Gall-Causing Agrobacterium spp.
Sign Up to like & getrecommendations! Published in 2017 at "Phytopathology"
DOI: 10.1094/phyto-04-17-0144-r
Abstract: Agrobacterium is a genus of soilborne gram-negative bacteria. Members carrying oncogenic plasmids can cause crown gall disease, which has significant economic costs, especially for the orchard and nursery industries. Early and rapid detection of pathogenic… read more here.
Keywords: detection; isothermal amplification; agrobacterium spp; agrobacterium ... See more keywords
The Ti Plasmid, Driver of Agrobacterium Pathogenesis.
Sign Up to like & getrecommendations! Published in 2023 at "Phytopathology"
DOI: 10.1094/phyto-11-22-0432-ia
Abstract: The phytopathogenic bacterium Agrobacterium tumefaciens causes crown gall disease in plants, characterized by the formation of tumor-like galls where wounds were present. Nowadays, however, the bacterium and its Ti (tumor-inducing) plasmid is better known as… read more here.
Keywords: plasmid driver; agrobacterium; agrobacterium pathogenesis; driver agrobacterium ... See more keywords
Agrobacterium cavarae sp. nov., isolated from maize (Zea mays L.) roots.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of systematic and evolutionary microbiology"
DOI: 10.1099/ijsem.0.004441
Abstract: A bacterial strain designated as RZME10T was isolated from a Zea mays L. root collected in Spain. Results of analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Agrobacterium… read more here.
Keywords: agrobacterium cavarae; agrobacterium; zea mays; cavarae nov ... See more keywords