Articles with "agrobacterium" as a keyword



Co-culturing on dry filter paper significantly increased the efficiency of Agrobacterium-mediated transformations of maize immature embryos

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Published in 2018 at "Physiology and Molecular Biology of Plants"

DOI: 10.1007/s12298-018-00641-5

Abstract: In the Agrobacterium tumefaciens-mediated transformations of maize immature embryos (IEs), the common co-culturing media used are MS or N6-based (MC). Here, we used a novel co-culturing method in which maize ‘Qi319’ IEs inoculated with Agrobacterium-harboring… read more here.

Keywords: maize immature; mediated transformations; transformations maize; agrobacterium ... See more keywords

Evaluation of the interspecific competition within Agrobacterium spp. in the soil and rhizosphere of tomato and maize

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Published in 2018 at "Journal of Plant Pathology"

DOI: 10.1007/s42161-018-0114-y

Abstract: Agrobacterium biovar 1 is responsible for crown gall disease of many plant species. However, according to the current species definition, this taxon displays too large a genomic divergence to be a single species. This is… read more here.

Keywords: plant; tomato maize; agrobacterium; soil rhizosphere ... See more keywords
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Agrobacterium strains and strain improvement: Present and outlook.

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Published in 2020 at "Biotechnology advances"

DOI: 10.1016/j.biotechadv.2020.107677

Abstract: Almost 40 years ago the first transgenic plant was generated through Agrobacterium tumefaciens-mediated transformation, which, until now, remains the method of choice for gene delivery into plants. Ever since, optimized Agrobacterium strains have been developed with… read more here.

Keywords: transformation; agrobacterium; agrobacterium strains; strain improvement ... See more keywords

Establishment of Agrobacterium tumefaciens - mediated genetic transformation of apple pathogen Marssonina coronaria using marker genes under the control of CaMV 35S promoter.

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Published in 2021 at "Microbiological research"

DOI: 10.1016/j.micres.2021.126878

Abstract: Premature leaf fall of apple caused by Marssonina coronaria is economically very important apple disease and all the commercially available apple cultivars are susceptible to this disease. The non-availability of an efficient transformation system for… read more here.

Keywords: camv 35s; transformation; 35s promoter; agrobacterium ... See more keywords

A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius.

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Published in 2017 at "Journal of microbiological methods"

DOI: 10.1016/j.mimet.2017.01.015

Abstract: In recent years, versatile genetic tools have been developed and applied to a number of filamentous fungi of industrial importance. However, the existing techniques have limitations when it comes to achieve the desired genetic modifications,… read more here.

Keywords: agrobacterium; gene targeting; aspergillus carbonarius; gene ... See more keywords
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Optimization of regeneration and Agrobacterium-mediated transformation of Stevia (Stevia rebaudiana Bertoni): a commercially important natural sweetener plant

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Published in 2020 at "Scientific Reports"

DOI: 10.1038/s41598-020-72751-8

Abstract: Stevia rebaudiana Bertoni is a commercially important zero calorie natural-sweetener herb which produce sweet compounds known as steviol glycosides. Rising demands of steviol glycosides by food and beverage industries has led to an increase in… read more here.

Keywords: stevia; stevia rebaudiana; bertoni commercially; transformation ... See more keywords

Agrobacterium virulence factors induce the expression of host DNA repair-related genes without promoting major genomic damage

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Published in 2024 at "Scientific Reports"

DOI: 10.1038/s41598-024-75525-8

Abstract: This study aimed to investigate whether the plant DNA damage levels and DNA damage response (DDR) are regulated during Agrobacterium infection and potentially manipulated by Agrobacterium to facilitate T-DNA integration. We investigated the plant genomic… read more here.

Keywords: host; damage; dna repair; agrobacterium ... See more keywords

Agrobacterium-Mediated Insect Resistance Gene (cry1Aa) Transfer Studies Pertaining to Antibiotic Sensitivity on Cultured Tissues of Broccoli

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Published in 2017 at "International Journal of Vegetable Science"

DOI: 10.1080/19315260.2017.1334734

Abstract: ABSTRACT Successful plant transformation requires an efficient regeneration protocol and a suitable selection method. A genetically engineered, disarmed Agrobacterium tumefaciens strain containing a binary vector pBin-1Aa with cry1Aa (insect resistance) and npt-II (neomycin phosphotransferase-II) genes… read more here.

Keywords: tissues broccoli; pertaining antibiotic; regeneration; insect resistance ... See more keywords

Isolation and characterization of the 2,5-pyridinedicarboxylic acid-degrading bacterium Agrobacterium sp. strain YJ-5.

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Published in 2023 at "Letters in applied microbiology"

DOI: 10.1093/lambio/ovac040

Abstract: 2,5-Pyridinedicarboxylic acid (2,5-PDA), a natural N-heterocyclic compound and a substitute for production in plastics, was widely distributed in industrial wastewater. However, the biodegradation of 2,5-PDA has been rarely reported. In this study, strain YJ-5, which… read more here.

Keywords: isolation characterization; agrobacterium; strain; pyridinedicarboxylic acid ... See more keywords

First report of crown gall on sweet cherry trees caused by Agrobacterium larrymoorei in South Korea

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Published in 2025 at "Plant Disease"

DOI: 10.1094/pdis-07-25-1460-pdn

Abstract: In October 2024, crown gall was observed in 4-year-old cherry trees (Prunus avium) in an orchard at Sancheong, Gyeongsangnam-do, South Korea. The gall affected approximately 10% of all 200 trees in the orchard (0.4 ha).… read more here.

Keywords: cherry trees; agrobacterium; crown gall; five isolates ... See more keywords

Isothermal Amplification and Lateral-Flow Assay for Detecting Crown-Gall-Causing Agrobacterium spp.

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Published in 2017 at "Phytopathology"

DOI: 10.1094/phyto-04-17-0144-r

Abstract: Agrobacterium is a genus of soilborne gram-negative bacteria. Members carrying oncogenic plasmids can cause crown gall disease, which has significant economic costs, especially for the orchard and nursery industries. Early and rapid detection of pathogenic… read more here.

Keywords: detection; isothermal amplification; agrobacterium spp; agrobacterium ... See more keywords