Effects of plant density on recombinant hemagglutinin yields in an Agrobacterium‐mediated transient gene expression system using Nicotiana benthamiana plants
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26303
Abstract: Agrobacterium‐mediated transient expression systems enable plants to rapidly produce a wide range of recombinant proteins. To achieve economically feasible upstream production and downstream processing, it is beneficial to obtain high levels of two yield‐related quantities… read more here.
Keywords: agrobacterium mediated; density; expression; mediated transient ... See more keywords
Agrobacterium-Mediated Seedling Transformation to Measure Circadian Rhythms in Arabidopsis.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1912-4_5
Abstract: Circadian clocks are endogenous timing mechanisms that allow an organism to adapt cellular processes in anticipation of predictable changes in the environment. Luciferase reporters are well utilized as an effective, nondestructive method to measure circadian… read more here.
Keywords: circadian rhythms; mediated seedling; agrobacterium mediated; measure circadian ... See more keywords
Agrobacterium-Mediated Transformation of Leaf Base Segments.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6682-0_7
Abstract: Agrobacterium-mediated transformation has become a routine method of genetic engineering of cereals, gradually replacing the biolistic protocols. Simple integration patterns of transgenic loci, decent transformation efficiency, and technical simplicity are the main advantages offered by… read more here.
Keywords: transformation leaf; agrobacterium mediated; transformation; leaf base ... See more keywords
Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7874-8_8
Abstract: Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency… read more here.
Keywords: mediated transformation; transformation efficiency; agrobacterium mediated; transformation ... See more keywords
An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize.
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DOI: 10.1007/978-1-4939-8991-1_10
Abstract: Precise genome engineering can be efficiently made using the revolutionary tool named CRISPR/Cas (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein) systems. Adapted from the bacterial immune system, CRISPR/Cas systems can generate highly specific double-strand breaks… read more here.
Keywords: crispr cas9; cas9; agrobacterium mediated; genome editing ... See more keywords
A tau class GST, OsGSTU5, interacts with VirE2 and modulates the Agrobacterium-mediated transformation in rice.
Sign Up to like & getrecommendations! Published in 2022 at "Plant cell reports"
DOI: 10.1007/s00299-021-02824-z
Abstract: KEY MESSAGE OsGSTU5 interacts and glutathionylates the VirE2 protein of Agrobacterium and its (OsGSTU5) overexpression and downregulation showed a low and high AMT efficiency in rice, respectively. During Agrobacterium-mediated transformation (AMT), T-DNA along with several… read more here.
Keywords: mediated transformation; vire2; agrobacterium mediated; rice ... See more keywords
Agrobacterium-mediated Tnt1 mutagenesis of moss protonemal filaments and generation of stable mutants with impaired gametophyte
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Genetics and Genomics"
DOI: 10.1007/s00438-019-01532-4
Abstract: The gametophyte of moss exhibits a simple body plan, yet its growth is regulated by complex developmental phenomena similar to angiosperms. Because moss can be easily maintained under laboratory conditions, amenable for gene targeting and… read more here.
Keywords: mediated tnt1; protonemal filaments; agrobacterium mediated; tnt1 mutagenesis ... See more keywords
Optimization of Agrobacterium-mediated transient expression of heterologous genes in spinach
Sign Up to like & getrecommendations! Published in 2017 at "Plant Biotechnology Reports"
DOI: 10.1007/s11816-017-0457-4
Abstract: The Agrobacterium-mediated transient assay is a relatively rapid technique and a promising approach for assessing the expression of a gene of interest. Despite the successful application of this transient expression system in several plant species,… read more here.
Keywords: transient expression; infiltration; agrobacterium mediated; expression ... See more keywords
The use of Agrobacterium-mediated insertional mutagenesis sequencing to identify novel genes of Humicola insolens involved in cellulase production
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1166-6
Abstract: A transfer DNA (T-DNA)-tagged mutant library of Humicola insolens was screened for mutants with altered cellulase production using the plate-clearing zone assay. Three selected mutants (5-A7, 5-C6, and 13-B7) exhibited significantly depressed FPase, CMCase and… read more here.
Keywords: production; humicola insolens; agrobacterium mediated; mediated insertional ... See more keywords
A protocol for functional study of genes in Brassica juncea by Agrobacterium-mediated transient expression: applicability in other Brassicaceae
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Biochemistry and Biotechnology"
DOI: 10.1007/s13562-019-00543-x
Abstract: Agrobacterium-mediated transient expression in plant organs is a quick and reliable method for studying gene functions. Due to the significance of transient transformation, substantial efforts have been dedicated to developing such protocols in various plants… read more here.
Keywords: transient expression; agrobacterium mediated; brassica juncea; mediated transient ... See more keywords
Genetic manipulation of Fonsecaea pedrosoi using particles bombardment and Agrobacterium mediated transformation.
Sign Up to like & getrecommendations! Published in 2018 at "Microbiological research"
DOI: 10.1016/j.micres.2018.01.001
Abstract: Fonsecaea pedrosoi, a melanized fungal pathogen that causes Chromoblastomycosis, a human disease with a worldwide distribution. Biolistic is a widely used technique for direct delivery of genetic material into intact cells by particles bombardment. Another… read more here.
Keywords: agrobacterium mediated; transformation; particles bombardment; fonsecaea ... See more keywords