Transformation of Campanula by wild type Agrobacterium rhizogenes
Sign Up to like & getrecommendations! Published in 2017 at "Euphytica"
DOI: 10.1007/s10681-017-1845-0
Abstract: Compact growth is an important quality criterion in horticulture. Many Campanula species and cultivars exhibit elongated growth which is suppressed by chemical retardation and cultural practice during production to accommodate to the consumer’s desire. The… read more here.
Keywords: transformation; agrobacterium rhizogenes; type agrobacterium; campanula ... See more keywords
Establishment of Agrobacterium rhizogenes-mediated hairy root transformation of Crocus sativus L.
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DOI: 10.1007/s13205-020-02626-2
Abstract: Efficient transformation system for genetic improvement is essential in Crocus sativus, as it lacks sexual reproduction . This is the first report wherein an efficient protocol is developed for the transformation of Crocus sativus L.… read more here.
Keywords: transformation; crocus sativus; agrobacterium rhizogenes; transformation crocus ... See more keywords
Agrobacterium rhizogenes-mediated RNAi of Tripterygium wilfordii and application for functional study of terpenoid biosynthesis pathway genes
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DOI: 10.1016/j.indcrop.2019.111509
Abstract: Abstract Tripterygium wilfordii is an important medical plants which is rich in several valuable bioactive terpenoids, such as triptolide and wilforine. Developing a fast and efficient transformation method is imperative for exploring these metabolites biosynthesis… read more here.
Keywords: biosynthesis pathway; tripterygium; tripterygium wilfordii; agrobacterium rhizogenes ... See more keywords
Fast track to obtain heritable transgenic sweet potato inspired by its evolutionary history as a naturally transgenic plant
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DOI: 10.1111/pbi.13986
Abstract: Sweet potato (Ipomoea batatas [L.] Lam.) is one of the most important crops in the world. Conventional genetic engineering requires delivering genetic changes to plant cells usually using Agrobacterium tumefaciens or Agrobacterium rhizogenes (Otani et… read more here.
Keywords: sweet potato; storage roots; agrobacterium rhizogenes; plant ... See more keywords
Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies
Sign Up to like & getrecommendations! Published in 2018 at "Plant Methods"
DOI: 10.1186/s13007-018-0315-6
Abstract: Background Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop.… read more here.
Keywords: chickpea; genetics; method; transformation ... See more keywords
Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus
Sign Up to like & getrecommendations! Published in 2021 at "Plant Methods"
DOI: 10.1186/s13007-021-00764-z
Abstract: Background Protocols for Agrobacterium rhizogenes -mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improve the transformation efficiency. Here,… read more here.
Keywords: lotus japonicus; transformation; agrobacterium rhizogenes; japonicus ... See more keywords
Small RNAs Derived from the T-DNA of Agrobacterium rhizogenes in Hairy Roots of Phaseolus vulgaris
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2017.00096
Abstract: Agrobacterium rhizogenes is a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically… read more here.
Keywords: hairy roots; art srnas; rhizogenes hairy; small rnas ... See more keywords
AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes–mediated transformation
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.952428
Abstract: Agrobacterium rhizogenes–mediated (ARM) transformation is an efficient and powerful tool to generate transgenic roots to study root-related biology. For loss-of-function studies, transgenic-root-induced indel mutations by CRISPR/Cas9 only with homozygous/biallelic mutagenesis can exhibit mutant phenotype(s) (excluding… read more here.
Keywords: promoter; homozygous biallelic; rhizogenes mediated; agrobacterium rhizogenes ... See more keywords