Articles with "aided amplification" as a keyword



Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.

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Published in 2018 at "Diagnostic microbiology and infectious disease"

DOI: 10.1016/j.diagmicrobio.2017.10.005

Abstract: In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The… read more here.

Keywords: rapid reverse; recombinase aided; transcription recombinase; aided amplification ... See more keywords

Rapid detection of Salmonella with Recombinase Aided Amplification.

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Published in 2017 at "Journal of microbiological methods"

DOI: 10.1016/j.mimet.2017.06.011

Abstract: Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice… read more here.

Keywords: recombinase aided; detection; salmonella; aided amplification ... See more keywords

Development of a Recombinase-Aided Amplification Assay for the Rapid Detection of Candida auris.

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Published in 2024 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.4c00450

Abstract: Candida auris (C. auris) was first discovered in Japan in 2009 and has since spread worldwide. It exhibits strong transmission ability, high multidrug resistance, blood infectivity, and mortality rates. Traditional diagnostic techniques for C. auris… read more here.

Keywords: detection; recombinase aided; aided amplification; clinical samples ... See more keywords

Recombinase-Aided Amplification Combined with Lateral Flow (LF-RAA) Assay for Rapid AAV Genome Detection

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Published in 2022 at "ACS Omega"

DOI: 10.1021/acsomega.2c05660

Abstract: Adeno-associated virus (AAV) is a versatile gene vector that is widely used in mammalian research. In basic studies and large-scale AAV production, genetic testing is ubiquitous and routine polymerase chain reaction (PCR)-based tests limit the… read more here.

Keywords: recombinase aided; aav genome; combined lateral; detection ... See more keywords

Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay

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Published in 2022 at "Frontiers in Cellular and Infection Microbiology"

DOI: 10.3389/fcimb.2022.922146

Abstract: Background Malaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders. Although these… read more here.

Keywords: using recombinase; recombinase aided; plasmodium; flow ... See more keywords

Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection

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Published in 2022 at "Frontiers in Microbiology"

DOI: 10.3389/fmicb.2022.928025

Abstract: Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification… read more here.

Keywords: psittaci; chlamydia psittaci; recombinase aided; infection ... See more keywords

Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

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Published in 2022 at "Frontiers in Veterinary Science"

DOI: 10.3389/fvets.2022.954657

Abstract: Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method… read more here.

Keywords: recombinase aided; detection method; transcription recombinase; detection ... See more keywords

Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay

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Published in 2023 at "Life"

DOI: 10.3390/life13020494

Abstract: Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic… read more here.

Keywords: recombinase aided; rapid onsite; detection; aided amplification ... See more keywords

Development and Application of Fluorescent and Lateral Flow Dipstick Recombinase-Aided Amplification for Rapid Detection of Glaesserella parasuis

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Published in 2025 at "Veterinary Sciences"

DOI: 10.3390/vetsci12080750

Abstract: Simple Summary Targeting the rpoB gene of Glaesserella parasuis, we developed the Recombinase-Aided Amplification with fluorescence (Fluo-RAA) or lateral flow dipstick (LFD-RAA) assays to detect Glaesserella parasuis in clinical samples. They are characterized by their… read more here.

Keywords: glaesserella; recombinase aided; glaesserella parasuis; aided amplification ... See more keywords