A unique AA5 alcohol oxidase fused with a catalytically inactive CE3 domain from the bacterium Burkholderia pseudomallei.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14632
Abstract: Copper radical oxidases (CROs) are redox enzymes able to oxidize alcohols or aldehydes, while only requiring a single copper atom as cofactor. Studied CROs are found in one of two subfamilies within the Auxiliary Activities… read more here.
Keywords: alcohol oxidase; burkholderia pseudomallei; domain;
An Engineered Alcohol Oxidase for the Oxidation of Primary Alcohols
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800556
Abstract: Structure‐guided directed evolution of choline oxidase has been carried out by using the oxidation of hexan‐1‐ol to hexanal as the target reaction. A six‐amino‐acid variant was identified with a 20‐fold increased kcat compared to that… read more here.
Keywords: oxidase; engineered alcohol; oxidation; alcohol oxidase ... See more keywords
Synthesis and characterization of Ogataea thermomethanolica alcohol oxidase immobilized on barium ferrite magnetic microparticles.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.08.007
Abstract: Alcohol oxidase catalyzes the oxidation of primary alcohols into the corresponding aldehydes, making it a potential biocatalyst in the chemical industry. However, the high production cost and poor operational stability of this enzyme are limitations… read more here.
Keywords: alcohol oxidase; ogataea thermomethanolica; enzyme; microscopy ... See more keywords
Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.12.012
Abstract: We cloned a set of genes encoding alcohol oxidase from Ochrobactrum sp. AIU 033 (OcAOD), which exhibits the appropriate substrate specificity for glyoxylic acid production from glycolic acid. The set of genes for OcAOD contained… read more here.
Keywords: ochrobactrum aiu; escherichia coli; alcohol oxidase; aiu 033 ... See more keywords
Enzymatic halogenation and oxidation using an alcohol oxidase-vanadium chloroperoxidase cascade
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Catalysis"
DOI: 10.1016/j.mcat.2017.09.014
Abstract: The chemo-enzymatic cascade which combines alcohol oxidase from Hansenula polymorpha (AOXHp) with vanadium chloroperoxidase (VCPO), for the production of biobased nitriles from amino acids was investigated. In the first reaction H2O2 (and acetaldehyde) are generated… read more here.
Keywords: aoxhp; cascade; alcohol oxidase; vanadium chloroperoxidase ... See more keywords
A rapid method to detect and estimate the activity of the enzyme, alcohol oxidase by the use of two chemical complexes - acetylacetone (3,5-diacetyl-1,4-dihydrolutidine) and acetylacetanilide (3,5-di-N-phenylacetyl-1,4-dihydrolutidine).
Sign Up to like & getrecommendations! Published in 2019 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2019.01.021
Abstract: A rapid and sensitive method has been devised in order to detect and estimate the synthesis of the enzyme alcohol oxidase (AOX) by fungi, by way of the use of two chemical complexes, namely, acetylacetone… read more here.
Keywords: phenylacetyl dihydrolutidine; dihydrolutidine; alcohol oxidase; diacetyl dihydrolutidine ... See more keywords
Reusable alcohol oxidase–nPtCu/alginate beads for highly sensitive ethanol assay in beverages
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra02106d
Abstract: Nanozymes (NZs) are nanoparticles that mimic the catalytic properties of natural enzymes. The present work aimed to obtain effective peroxidase mimetics (PO-like NZs), to characterize their morphological properties, estimate the kinetic parameters of NZs and… read more here.
Keywords: alginate beads; alcohol oxidase; reusable alcohol; nptcu alginate ... See more keywords
A novel amperometric bienzymatic biosensor based on alcohol oxidase coupled PVC reaction cell and nanomaterials modified working electrode for rapid quantification of alcohol
Sign Up to like & getrecommendations! Published in 2018 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2018.1514515
Abstract: Abstract A new amperometric sensor has been fabricated for sensitive and rapid quantification of ethanol. The biosensor assembly was prepared by covalently immobilizing alcohol oxidase (AOX) from Pichia pastoris onto chemically modified surface of polyvinylchloride… read more here.
Keywords: biosensor; alcohol oxidase; alcohol; rapid quantification ... See more keywords
Oxidation of 5-hydroxymethylfurfural with a novel aryl alcohol oxidase from Mycobacterium sp. MS1601.
Sign Up to like & getrecommendations! Published in 2022 at "Microbial biotechnology"
DOI: 10.1111/1751-7915.14052
Abstract: Bio-based 5-hydroxymethylfurfural (HMF) serves as an important platform for several chemicals, among which 2,5-furan dicarboxylic acid (FDCA) has attracted considerable interest as a monomer for the production of polyethylene furanoate (PEF), a potential alternative for… read more here.
Keywords: oxidation; alcohol oxidase; mycobacterium; aryl alcohol ... See more keywords
Properties, Physiological Functions and Involvement of Basidiomycetous Alcohol Oxidase in Wood Degradation
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232213808
Abstract: Extensive research efforts have been devoted to describing yeast alcohol oxidase (AO) and its promoter region, which is vastly applied in studies of heterologous gene expression. However, little is known about basidiomycetous AO and its… read more here.
Keywords: alcohol; degradation; alcohol oxidase; wood degradation ... See more keywords