Extraction and characterization of alkaline protease from Streptomyces sp. GS-1 and its application as dehairing agent
Sign Up to like & getrecommendations! Published in 2020 at "Biocatalysis and agricultural biotechnology"
DOI: 10.1016/j.bcab.2020.101590
Abstract: Abstract Several microbial proteases were reported for dehairing; however, they become unstable and lose the activity at alkaline conditions during the process. Hence, there is a need for protease that withstands its activity at high… read more here.
Keywords: protease; characterization alkaline; alkaline protease; alkaline ... See more keywords
Nematicidal activity of thermostable alkaline protease produced by Saccharomonospora viridis strain Hw G550
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Reports"
DOI: 10.1016/j.btre.2019.e00386
Abstract: Highlights • Isolation and identification of thermo alkaliphilic actinomycetes.• Obtaining of thermostable alkaline protease enzyme.• Evaluation of the nematicidal activity of obtained protease.• Application of thermostable alkaline protease as nemticidal agent. read more here.
Keywords: nematicidal activity; protease; thermostable alkaline; activity thermostable ... See more keywords
Cloning, expression and characterization of a novel alkaline serine protease gene from native Iranian Bacillus sp.; a producer of protease for use in livestock.
Sign Up to like & getrecommendations! Published in 2019 at "Gene"
DOI: 10.1016/j.gene.2019.01.020
Abstract: The use of proteases in the last decade has been welcomed in livestock and poultry industries and has led to significant results such as improved feed conversion ratio, weight gain and increased growth performance. In… read more here.
Keywords: protease; use; gene; alkaline protease ... See more keywords
Development and characterization of cross-linked enzyme aggregates of thermotolerant alkaline protease from Bacillus licheniformis.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.03.009
Abstract: An alkaline protease was produced by B. licheniformis with 132.43±3.4U/mL activity in LSF which was further enhanced by optimizing culture conditions. The optimum enzyme activity (148.9±4.1U/mL) was harvested at pH7.5; temperature, 40°C and inoculum, 1.5mL… read more here.
Keywords: enzyme aggregates; protease; linked enzyme; cross linked ... See more keywords
Development of nano-silver alkaline protease bio-conjugate depilating eco-benign formulation by utilizing potato peel based medium.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.02.251
Abstract: A new bio-conjugate nano-silver enzyme conjugate complex (BC-nAg-Akp) was formulated containing alkaline protease (Akp). The present research involved synthesis of nAg particles in acetone concentrated enzyme sol using 0.005 M AgNO3 solution formed within interaction time… read more here.
Keywords: nano silver; conjugate; formulation; bio conjugate ... See more keywords
Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.07.266
Abstract: A new serine alkaline protease (designated as SAPGB) from Gracilibacillus boraciitolerans LO15, was produced (9000 U/mL), purified, and characterized. SAPGB has a monomer structure with a precise molecular weight of 30,285.03 Da as learnt from matrix-assisted laser… read more here.
Keywords: gracilibacillus boraciitolerans; serine alkaline; protease; homology modeling ... See more keywords
High-level expression, purification, and enzymatic characterization of a recombinant Aspergillus sojae alkaline protease in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.03.009
Abstract: An alkaline protease (Ap) was cloned from Aspergillus sojae GIM3.33 via RT-PCR technique. A truncated Ap without the signal peptide was successfully expressed in the Pichia pastoris KM71 strain. The following describes the optimal process… read more here.
Keywords: expression purification; rap; pichia pastoris; alkaline protease ... See more keywords
A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches
Sign Up to like & getrecommendations! Published in 2019 at "Industrial Biotechnology"
DOI: 10.1089/ind.2018.0032
Abstract: Abstract Proteolytic enzymes are present in all living organisms and help in cell growth and differentiation. Proteases are the hydrolytic enzymes that act as biocatalysts for the cleavage of prote... read more here.
Keywords: tool various; protease essential; microbial alkaline; essential tool ... See more keywords
Effect of Proteolysis with Alkaline Protease Following High Hydrostatic Pressure Treatment on IgE Binding of Buckwheat Protein.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of food science"
DOI: 10.1111/1750-3841.13627
Abstract: Buckwheat is a popular food material in many Asian countries and it contains major allergenic proteins. This study was performed to analyze the effects of hydrolysis with alkaline protease following high hydrostatic pressure (HHP) treatment… read more here.
Keywords: binding buckwheat; ige binding; buckwheat; alkaline protease ... See more keywords
Antibodies Against Pseudomonas aeruginosa Alkaline Protease Directly Enhance Disruption of Neutrophil Extracellular Traps Mediated by This Enzyme
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2021.654649
Abstract: Extracellular traps released by neutrophils (NETs) are essential for the clearance of Pseudomonas aeruginosa. Alkaline protease (AprA) secreted by P. aeruginosa negatively correlates with clinical improvement. Moreover, anti-AprA in patients with cystic fibrosis (CF) can… read more here.
Keywords: aeruginosa alkaline; pseudomonas aeruginosa; apra; extracellular traps ... See more keywords
Characterization of redox and salinity-tolerant alkaline protease from Bacillus halotolerans strain DS5
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.935072
Abstract: Bacillus halotolerans DS5 was isolated and identified as a halophilic microbe according to 16S rRNA analysis and the physical and chemical indices of the strain. A new alkaline protease (designated as prot DS5) from Bacillus… read more here.
Keywords: prot ds5; bacillus halotolerans; alkaline protease;