An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species
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DOI: 10.1186/s13568-017-0425-y
Abstract: Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones… read more here.
Keywords: genome editing; vector free; allele exchange; protocol ... See more keywords