Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202400280
Abstract: D‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase). To… read more here.
Keywords: thermostability; semi rational; engineering allulose; allulose epimerase ... See more keywords
Characterization of a recombinant d-allulose 3-epimerase from Agrobacterium sp. ATCC 31749 and identification of an important interfacial residue.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.02.036
Abstract: d-Allulose 3-epimerase (DAEase) catalyzes the epimerization between d-fructose and d-allulose. We had PCR-cloned and overexpressed the gene encoding Agrobacterium sp. ATCC 31749 DAEase (AsDAEase) in Escherichia coli. A high yield of active AsDAEase, 35,300U/L or… read more here.
Keywords: atcc 31749; allulose epimerase; agrobacterium atcc; asdaease ... See more keywords
Computer-Aided Targeted Mutagenesis of Thermoclostridium caenicola d-Allulose 3-Epimerase for Improved Thermostability.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c07256
Abstract: d-Allulose 3-epimerase (DAEase) is a key enzyme in d-allulose bioproduction. DAEase from Thermoclostridium caenicola suffers from poor thermostability, hampering its large-scale applications in industry. In this study, mutants A70P, G107P, F155Y, and D162T with increased… read more here.
Keywords: improved thermostability; thermoclostridium caenicola; allulose epimerase; targeted mutagenesis ... See more keywords
Boosting the Heterologous Expression of d-Allulose 3-Epimerase in Bacillus subtilis through Protein Engineering and Catabolite-Responsive Element Box Engineering.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c04800
Abstract: As a natural sweetener with low calories and various physiological activities, d-allulose has drawn worldwide attention. Currently, d-allulose 3-epimerase (DAEase) is mainly used to catalyze the epimerization of d-fructose to d-allulose. Therefore, it is quite… read more here.
Keywords: daease; bacillus subtilis; allulose epimerase; expression ... See more keywords
Enhancing Stability and Catalytic Activity of d-Allulose 3-Epimerase through Multistrategy Computational Design and Cross-Regional Advantageous Mutations.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c07342
Abstract: d-Allulose 3-epimerase (DAEase) derived from Clostridium bolteae has excellent properties in the catalytic production of d-allulose, a rare sugar with unique biological functions. However, the industrial application of C. bolteae DAEase (Cb-DAEase) for d-allulose production… read more here.
Keywords: cross regional; allulose epimerase; enzyme activity; design ... See more keywords
Coexpression of D-Allulose 3-Epimerase and L-Rhamnose Isomerase in Bacillus subtilis through a Dual Promoter Enables High-Level Biosynthesis of D-Allose from D-Fructose in One Pot.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c09787
Abstract: D-Allose, a rare sugar, has gained significant attention not only as a low-calorie sweetener but also for its anticancer, antitumor, anti-inflammatory, antioxidant, and other pharmaceutical properties. Despite its potential, achieving high-level biosynthesis of D-allose remains… read more here.
Keywords: level biosynthesis; rhamnose isomerase; bacillus subtilis; high level ... See more keywords
Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Cell Factories"
DOI: 10.1186/s12934-022-01789-2
Abstract: Background D-allulose, a hexulose monosaccharide with low calorie content and high sweetness, is commonly used as a functional sugar in food and nutrition. However, enzyme preparation of D-allulose from D-frutose was severely hindered by the… read more here.
Keywords: expression; synthesis; tandem allulose; allulose epimerase ... See more keywords
Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application
Sign Up to like & getrecommendations! Published in 2025 at "PLOS One"
DOI: 10.1371/journal.pone.0327420
Abstract: D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a… read more here.
Keywords: induction; allulose epimerase; daease; production ... See more keywords
Characterization of D-Allulose-3-Epimerase From Ruminiclostridium papyrosolvens and Immobilization Within Metal-Organic Frameworks
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.869536
Abstract: D-allulose is one sort of C-3 epimer of D-fructose with the low calorie (0.4 kcal/g) and high sweetness (70% of the relative sweetness of sucrose), which can be biosynthesized by D-allulose-3-epimerase (DAE). In this work,… read more here.
Keywords: allulose epimerase; characterization allulose; ruminiclostridium papyrosolvens; epimerase ruminiclostridium ... See more keywords
Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12111547
Abstract: d-Allulose is the corresponding epimer of d-fructose at the C-3 position, which exhibits a similar taste and sweetness to sucrose. As a low-calorie sweetener, d-allulose has broad application prospects in the fields of medicine, food,… read more here.
Keywords: daease; screening assay; allulose epimerase; throughput screening ... See more keywords
The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability
Sign Up to like & getrecommendations! Published in 2024 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms25126361
Abstract: As a low-calorie sugar, D-allulose is produced from D-fructose catalyzed by D-allulose 3-epimerase (DAE). Here, to improve the catalytic activity, stability, and processability of DAE, we reported a novel method by forming organic–inorganic hybrid nanoflowers… read more here.
Keywords: activity stability; dae; daes; allulose epimerase ... See more keywords