Structural and biochemical characterization of the Clostridium perfringens-specific Zn2+-dependent amidase endolysin, Psa, catalytic domain.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.08.085
Abstract: Phage-derived endolysins, enzymes that degrade peptidoglycans, have the potential to serve as alternative antimicrobial agents. Psa, which was identified as an endolysin encoded in the genome of Clostridium perfringens st13, was shown to specifically lyse… read more here.
Keywords: psa catalytic; catalytic domain; clostridium perfringens; amidase ... See more keywords
Staphylococcus aureus cell growth and division are regulated by an amidase that trims peptides from uncrosslinked peptidoglycan
Sign Up to like & getrecommendations! Published in 2020 at "Nature microbiology"
DOI: 10.1038/s41564-019-0632-1
Abstract: Bacteria are protected by a polymer of peptidoglycan that serves as an exoskeleton1. In Staphylococcus aureus, the peptidoglycan assembly enzymes relocate during the cell cycle from the periphery, where they are active during growth, to… read more here.
Keywords: division; cell growth; amidase; growth division ... See more keywords
An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division
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DOI: 10.1038/s41598-017-01184-7
Abstract: Mycobacteria possess a multi-layered cell wall that requires extensive remodelling during cell division. We investigated the role of an amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase, a peptidoglycan remodelling enzyme implicated in cell division. We demonstrated that deletion… read more here.
Keywords: cell division; amidase; cell wall; amidase domain ... See more keywords
Computational design of an amidase by combining the best electrostatic features of two promiscuous hydrolases
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d2sc00778a
Abstract: While there has been emerging interest in designing new enzymes to solve practical challenges, computer-based options to redesign catalytically active proteins are rather limited. Here, a rational QM/MM molecular dynamics strategy based on combining the… read more here.
Keywords: amidase; best electrostatic; computational design; combining best ... See more keywords
Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1101/2022.01.21.477235
Abstract: Construction and remodeling of the bacterial peptidoglycan (PG) cell wall must be carefully coordinated with cell growth and division. Central to cell wall construction are hydrolases that cleave bonds in peptidoglycan. These enzymes also represent… read more here.
Keywords: cell wall; metal cofactor; amidase; partner ... See more keywords
The Plant Growth-Promoting Rhizobacterium Variovorax boronicumulans CGMCC 4969 Regulates the Level of Indole-3-Acetic Acid Synthesized from Indole-3-Acetonitrile
Sign Up to like & getrecommendations! Published in 2018 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00298-18
Abstract: We demonstrated that Variovorax boronicumulans CGMCC 4969 has two enzymatic systems—nitrilase and nitrile hydratase/amidase—that convert indole-3-acetonitrile (IAN) to the important plant hormone indole-3-acetic acid (IAA). The two IAA synthesis systems have very different regulatory mechanisms,… read more here.
Keywords: plant; boronicumulans cgmcc; amidase; iaa synthesis ... See more keywords
The Novel Amidase PcnH Initiates the Degradation of Phenazine-1-Carboxamide in Sphingomonas histidinilytica DS-9
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00543-22
Abstract: Phenazines have been widely acknowledged as a natural antibiotic for more than 150 years, but their degradation mechanisms are still not completely elucidated. Compared with the studies on the degradation mechanism of PCA and PYO, little… read more here.
Keywords: pcnh; amidase; pca; pcn ... See more keywords
Pigmentiphaga sp. Strain D-2 Uses a Novel Amidase To Initiate the Catabolism of the Neonicotinoid Insecticide Acetamiprid
Sign Up to like & getrecommendations! Published in 2020 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.02425-19
Abstract: Acetamiprid, among the top neonicotinoid insecticides used worldwide, is one of the most important commercial insecticides. Due to its extensive use, the environmental fate of acetamiprid, especially its microbial degradation, has caused considerable concern. Although… read more here.
Keywords: aceab; pigmentiphaga strain; amidase; novel amidase ... See more keywords
A Novel Chimeric Endolysin with Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2017.00290
Abstract: Cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) and amidase are known as catalytic domains of the bacteriophage-derived endolysin LysK and were previously reported to show lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). In the current study, the in silico… read more here.
Keywords: antibacterial activity; chap amidase; chimeric chap; amidase ... See more keywords
A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm
Sign Up to like & getrecommendations! Published in 2021 at "Antibiotics"
DOI: 10.3390/antibiotics10030245
Abstract: Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential treatments include phage-derived endolysins, a promising family of alternative antimicrobial agents. We surveyed the genome… read more here.
Keywords: perfringens st13; clostridium perfringens; specific lytic; amidase ... See more keywords
Inhibition of L. monocytogenes Biofilm Formation by the Amidase Domain of the Phage vB_LmoS_293 Endolysin
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DOI: 10.3390/v11080722
Abstract: Listeria monocytogenes is a ubiquitous Gram-positive bacterium that is a major concern for food business operators because of its pathogenicity and ability to form biofilms in food production environments. Bacteriophages (phages) have been evaluated as… read more here.
Keywords: phage lmos; phage; domain phage; amidase ... See more keywords