Improving thermostability of (R)‐selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1572
Abstract: To improve the thermostability of (R)‐selective amine transaminase from Aspergillus terreus (AT‐ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were… read more here.
Keywords: selective amine; disulfide bonds; thermostability; amine transaminase ... See more keywords
Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor‐Binding Element of Amine Transaminase
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DOI: 10.1002/cbic.201700236
Abstract: Amine transaminase (ATA) catalyse enantioselectively the direct amination of ketones, but insufficient stability during catalysis limits their industrial applicability. Recently, we revealed that ATAs suffer from substrate‐induced inactivation mechanism involving dissociation of the enzyme–cofactor intermediate.… read more here.
Keywords: engineering cofactor; amine transaminase; binding element; stability ... See more keywords
Insight into the dimer dissociation process of the Chromobacterium violaceum (S)-selective amine transaminase
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DOI: 10.1038/s41598-019-53177-3
Abstract: One of the main factors hampering the implementation in industry of transaminase-based processes for the synthesis of enantiopure amines is their often low storage and operational stability. Our still limited understanding of the inactivation processes… read more here.
Keywords: dimer dissociation; selective amine; amine transaminase; chromobacterium violaceum ... See more keywords
Non-Canonical Amino Acid-Based Engineering of (R)-Amine Transaminase
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.839636
Abstract: Non-canonical amino acids (ncAAs) have been utilized as an invaluable tool for modulating the active site of the enzymes, probing the complex enzyme mechanisms, improving catalytic activity, and designing new to nature enzymes. Here, we… read more here.
Keywords: amino acid; non canonical; canonical amino; amine ... See more keywords