Virus genotyping by massive parallel amplicon sequencing: adenovirus and enterovirus in the Norwegian MIDIA study
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Medical Virology"
DOI: 10.1002/jmv.25361
Abstract: Direct genotyping of adenovirus or enterovirus from clinical material using polymerase chain reaction (PCR) followed by Sanger sequencing is often difficult due to the presence of multiple virus types in a sample, or due to… read more here.
Keywords: parallel amplicon; massive parallel; amplicon sequencing; adenovirus enterovirus ... See more keywords
Microbiome Analysis Using 16S Amplicon Sequencing: From Samples to ASVs.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1103-6_7
Abstract: In this chapter, we will present an outline of a typical experimental and bioinformatic workflow for identification of bacterial amplicon sequence variants (ASVs) present in a set of samples. This chapter is written from a… read more here.
Keywords: analysis; microbiome analysis; using 16s; 16s amplicon ... See more keywords
Effect of Amplicon Sequencing Depth in Environmental Microbiome Research.
Sign Up to like & getrecommendations! Published in 2021 at "Current microbiology"
DOI: 10.1007/s00284-021-02345-8
Abstract: Amplicon sequencing approach is commonly employed in microbiome studies and sequencing depth is considered as a major factor influencing the outcome of data analyses. As of now, the effect of amplicon sequencing depth in environmental… read more here.
Keywords: effect amplicon; data analyses; environmental microbiome; microbiome ... See more keywords
Targeted amplicon sequencing of 40 nuclear genes supports a single introduction and rapid radiation of Hawaiian Metrosideros (Myrtaceae)
Sign Up to like & getrecommendations! Published in 2019 at "Plant Systematics and Evolution"
DOI: 10.1007/s00606-019-01615-0
Abstract: Compared to traditionally used plastid or ribosomal markers, nuclear gene markers provide many advantages for molecular systematics of plants, and high-throughput sequencing technologies are making large nuclear datasets available at an unprecedented rate. We used… read more here.
Keywords: nuclear genes; hawaiian metrosideros; sequencing nuclear; targeted amplicon ... See more keywords
Influence of sampling and DNA extraction on 16S rRNA gene amplicon sequencing - Comparison of the bacterial community between two food processing plants
Sign Up to like & getrecommendations! Published in 2018 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2017.12.027
Abstract: Abstract 16S rRNA amplicon sequencing is frequently used to investigate and compare microbiomes. This study focuses on the potential, applicability and limits of 16S rRNA amplicon sequencing for comparing the microbiomes of two dairy production… read more here.
Keywords: extraction; dna extraction; 16s rrna; influence sampling ... See more keywords
Blastocystis subtype distribution in domestic and captive wild bird species from Brazil using next generation amplicon sequencing
Sign Up to like & getrecommendations! Published in 2020 at "Parasite Epidemiology and Control"
DOI: 10.1016/j.parepi.2020.e00138
Abstract: Blastocystis is a food and water borne intestinal parasite commonly identified in humans and many other animals worldwide. Of the nine potentially zoonotic subtypes of Blastocystis, seven have been reported in bird species. However molecular… read more here.
Keywords: generation amplicon; next generation; blastocystis subtype; amplicon sequencing ... See more keywords
Distinct relationships between fluorescence in situ hybridization and 16S rRNA gene- and amplicon-based sequencing data of bacterioplankton lineages.
Sign Up to like & getrecommendations! Published in 2019 at "Systematic and applied microbiology"
DOI: 10.1016/j.syapm.2019.06.005
Abstract: Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) and amplicon sequencing of the total (16S rRNA gene) and potentially active (16S rRNA transcripts), community are the major state of the art approaches for assessing the… read more here.
Keywords: sequencing data; bacterioplankton; amplicon; amplicon sequencing ... See more keywords
Superior resolution characterisation of microbial diversity in anaerobic digesters using full-length 16S rRNA gene amplicon sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Water research"
DOI: 10.1016/j.watres.2020.115815
Abstract: In the past decade, the characterisation of the microbial community in anaerobic digestion was primarily done by using high-throughput short-read amplicon sequencing. However, the short-read approach has inherent primer bias and low phylogenetic resolution. Our… read more here.
Keywords: resolution; characterisation microbial; full length; 16s rrna ... See more keywords
Amplicon sequencing provides more accurate microbiome information in healthy children compared to culturing
Sign Up to like & getrecommendations! Published in 2019 at "Communications Biology"
DOI: 10.1038/s42003-019-0540-1
Abstract: Next-Generation Sequencing (NGS) of 16S rRNA gene is now one of the most widely used application to investigate the microbiota at any given body site in research. Since NGS is more sensitive than traditional culture… read more here.
Keywords: culturing amplicon; provides accurate; sequencing provides; accurate microbiome ... See more keywords
Preclinical workup using long-read amplicon sequencing provides families with de novo pathogenic variants access to universal preimplantation genetic testing.
Sign Up to like & getrecommendations! Published in 2023 at "Human reproduction"
DOI: 10.1093/humrep/deac273
Abstract: STUDY QUESTION Can long-read amplicon sequencing be beneficial for preclinical preimplantation genetic testing (PGT) workup in couples with a de novo pathogenic variant in one of the prospective parents? SUMMARY ANSWER Long-read amplicon sequencing represents… read more here.
Keywords: pgt; universal pgt; amplicon sequencing; read amplicon ... See more keywords
Amplicon Sequencing Reveals Extensive Coinfections of Foliar Pathogens in Soybean.
Sign Up to like & getrecommendations! Published in 2020 at "Plant disease"
DOI: 10.1094/pdis-06-20-1165-re
Abstract: Soybean (Glycine max) is one of the most economically important crops grown in North America and in other regions worldwide. However, the plant is susceptible to a variety of foliar pathogenic microorganisms, some of which… read more here.
Keywords: reveals extensive; extensive coinfections; sequencing reveals; foliar pathogens ... See more keywords