Association between the Amplification Parameters of the α‐Synuclein Seed Amplification Assay and Clinical and Genetic Subtypes of Parkinson's Disease
Sign Up to like & getrecommendations! Published in 2024 at "Movement Disorders"
DOI: 10.1002/mds.30085
Abstract: α‐Synuclein seed amplification assay on cerebrospinal fluid (CSF‐αSyn‐SAA) has shown high accuracy for Parkinson's disease (PD) diagnosis. The analysis of CSF‐αSyn‐SAA parameters may provide useful insight to dissect the heterogeneity of synucleinopathies. read more here.
Keywords: synuclein seed; seed; seed amplification; amplification assay ... See more keywords
Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.734987
Abstract: Abstract Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about… read more here.
Keywords: amplification assay; detection; polymerase amplification; enterocytozoon hepatopenaei ... See more keywords
Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2019.05.001
Abstract: A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per… read more here.
Keywords: loop mediated; amplification assay; mediated isothermal; vibrio cholerae ... See more keywords
An enhanced isothermal amplification assay for viral detection
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DOI: 10.1038/s41467-020-19258-y
Abstract: Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development… read more here.
Keywords: amplification assay; detection; isothermal amplification; assay viral ... See more keywords
Development and evaluation of real‐time recombinase polymerase amplification assay for rapid and sensitive detection of Vibro mimicus in human plasma samples
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Applied Microbiology"
DOI: 10.1111/jam.15666
Abstract: We aimed at developing a fast and accurate method to detect Vibrio mimicus using real‐time recombinase polymerase amplification assay. read more here.
Keywords: amplification assay; time recombinase; mimicus; real time ... See more keywords
Seeding amplification assay: Limitations and insights for enhanced clinical and research applications
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Parkinson's Disease"
DOI: 10.1177/1877718x251325124
Abstract: The accurate diagnosis of synucleinopathies—neurodegenerative diseases marked by misfolded α-synuclein protein aggregates, such as Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy—remains a critical challenge. Conventional clinical criteria, frequently verified only through postmortem… read more here.
Keywords: diagnosis; disease; saa; seeding amplification ... See more keywords
Accurate detection of pathologic α-synuclein in CSF, skin, olfactory mucosa, and urine with a uniform seeding amplification assay
Sign Up to like & getrecommendations! Published in 2025 at "Acta Neuropathologica Communications"
DOI: 10.1186/s40478-025-02034-8
Abstract: Currently, early diagnosis of dementia with Lewy bodies (DLB) is based on clinical criteria, which is challenging due to overlapping symptoms with other neurodegenerative diseases. Seeding amplification assays, detecting minute amounts of disease causing α-synuclein… read more here.
Keywords: detection; olfactory mucosa; csf; amplification assay ... See more keywords
Development of a novel loop-mediated isothermal amplification assay for rapid detection of Mycobacterium leprae in clinical samples.
Sign Up to like & getrecommendations! Published in 2021 at "Indian journal of dermatology, venereology and leprology"
DOI: 10.25259/ijdvl_248_19
Abstract: BACKGROUND Sensitive and definitive diagnostic tests are required for timely treatment of leprosy and to control its transmission. AIM In the present study, we report the development of loop-mediated isothermal amplification assay using six primers… read more here.
Keywords: isothermal amplification; amplification assay; loop mediated; mediated isothermal ... See more keywords
Loop-mediated isothermal amplification assay for the rapid detection of Anaplasma marginale in cattle based on major surface protein 5 gene
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Vector Borne Diseases"
DOI: 10.4103/0972-9062.353249
Abstract: Background & objectives: Timely intervention is needed to minimize the economic losses of vector-borne bovine anaplasmosis which can be possible by the isothermal amplification assay. Methods: Anaplasma marginale in the cattle of south Gujarat, India… read more here.
Keywords: amplification assay; marginale cattle; isothermal amplification; detection ... See more keywords