Articles with "amplification assay" as a keyword



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Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay

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Published in 2020 at "Aquaculture"

DOI: 10.1016/j.aquaculture.2020.734987

Abstract: Abstract Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about… read more here.

Keywords: amplification assay; detection; polymerase amplification; enterocytozoon hepatopenaei ... See more keywords
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Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139.

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Published in 2019 at "Molecular and cellular probes"

DOI: 10.1016/j.mcp.2019.05.001

Abstract: A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per… read more here.

Keywords: loop mediated; amplification assay; mediated isothermal; vibrio cholerae ... See more keywords
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An enhanced isothermal amplification assay for viral detection

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Published in 2020 at "Nature Communications"

DOI: 10.1038/s41467-020-19258-y

Abstract: Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development… read more here.

Keywords: amplification assay; detection; isothermal amplification; assay viral ... See more keywords
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Development and evaluation of real‐time recombinase polymerase amplification assay for rapid and sensitive detection of Vibro mimicus in human plasma samples

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Published in 2022 at "Journal of Applied Microbiology"

DOI: 10.1111/jam.15666

Abstract: We aimed at developing a fast and accurate method to detect Vibrio mimicus using real‐time recombinase polymerase amplification assay. read more here.

Keywords: amplification assay; time recombinase; mimicus; real time ... See more keywords
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Development of a novel loop-mediated isothermal amplification assay for rapid detection of Mycobacterium leprae in clinical samples.

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Published in 2021 at "Indian journal of dermatology, venereology and leprology"

DOI: 10.25259/ijdvl_248_19

Abstract: BACKGROUND Sensitive and definitive diagnostic tests are required for timely treatment of leprosy and to control its transmission. AIM In the present study, we report the development of loop-mediated isothermal amplification assay using six primers… read more here.

Keywords: isothermal amplification; amplification assay; loop mediated; mediated isothermal ... See more keywords
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Loop-mediated isothermal amplification assay for the rapid detection of Anaplasma marginale in cattle based on major surface protein 5 gene

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Published in 2023 at "Journal of Vector Borne Diseases"

DOI: 10.4103/0972-9062.353249

Abstract: Background & objectives: Timely intervention is needed to minimize the economic losses of vector-borne bovine anaplasmosis which can be possible by the isothermal amplification assay. Methods: Anaplasma marginale in the cattle of south Gujarat, India… read more here.

Keywords: amplification assay; marginale cattle; isothermal amplification; detection ... See more keywords