Articles with "amplification cloning" as a keyword



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An efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic RNA segments.

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Published in 2017 at "Journal of virological methods"

DOI: 10.1016/j.jviromet.2016.12.018

Abstract: Tospoviruses (genus Tospovirus, family Bunyaviridae) are responsible for major losses in an extensive range of crops worldwide. New species of these single-stranded, ambisense RNA viruses regularly emerge and have been shown to maintain heterogeneous populations… read more here.

Keywords: tospovirus; genomic rna; rna; genome segments ... See more keywords