CtNorm: Real time PCR cycle of threshold (Ct) normalization algorithm.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106267
Abstract: In relative quantification with Real Time PCR (qRT-PCR,), accurate analysis requires equal amplification efficiency for both genes (Gene of interest and reference gene) and equal threshold values for all the samples. In this quantification method… read more here.
Keywords: algorithm; amplification efficiency; real time; time pcr ... See more keywords
Amplification Efficiency and Template Accessibility as Distinct Causes of Rain in Digital PCR: Monte Carlo Modeling and Experimental Validation.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03534
Abstract: Partitions in digital PCR (dPCR) assays do not reach the detection threshold at the same time. This heterogeneity in amplification results in intermediate endpoint fluorescence values (i.e., rain) and misclassification of partitions, which has a… read more here.
Keywords: template accessibility; amplification efficiency; rain; efficiency template ... See more keywords
Robust and Precise Quantitative Real-Time Polymerase Chain Reaction with an Amplification Efficiency-Aware Reaction Kinetics Model.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00061
Abstract: Quantitative real-time PCR (qPCR) is a method extensively used in nucleic acid testing for plants and animals. During the coronavirus disease 2019 (COVID-19) pandemic, high-precision qPCR analysis was urgently needed since quantitative results obtained from… read more here.
Keywords: reaction; amplification efficiency; reaction kinetics; kinetics model ... See more keywords