Detailed analysis of HTT repeat elements in human blood using targeted amplification‐free long‐read sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Human Mutation"
DOI: 10.1002/humu.23580
Abstract: Amplification of DNA is required as a mandatory step during library preparation in most targeted sequencing protocols. This can be a critical limitation when targeting regions that are highly repetitive or with extreme guanine–cytosine (GC)… read more here.
Keywords: repeat elements; analysis; repeat; amplification free ... See more keywords
Amplification-free detection of microRNAs via a rapid microarray-based sandwich assay
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0298-6
Abstract: The detection and profiling of microRNAs are of great interest in disease diagnosis and prognosis. In this paper, we present a method for the rapid amplification-free detection of microRNAs from total RNA samples. In a… read more here.
Keywords: detection; sandwich assay; free detection; amplification free ... See more keywords
Nucleic acid amplification-free detection of DNA and RNA at ultralow concentration.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2021.07.022
Abstract: The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10-18 M) concentration and lower is reviewed. These low concentrations correspond roughly to the most clinically desirable detection range… read more here.
Keywords: detection; free detection; amplification free; acid amplification ... See more keywords
Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02283
Abstract: CRISPR/Cas-based systems are highly attractive for developing next-generation diagnostic technologies because of their intrinsic merits such as simplicity, sensitivity, and specificity. However, currently, nucleic acid amplification procedures are still needed to achieve attomolar sensitivity in… read more here.
Keywords: dna; one step; amplification; single microbead ... See more keywords
Glycan-Evocated Metallization for Amplification-Free Electrochemical Detection of Glycoproteins at Low Concentration Levels.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c04054
Abstract: Glycoproteins have become the most often screened tumor markers in the in vitro diagnostics. Although a large number of electrochemical methods have been proposed to sensitively detect glycoproteins, most of them involve the aid of… read more here.
Keywords: detection; detection glycoproteins; metallization; glycan evocated ... See more keywords
Photo-Nanozyme-Integrated Photoelectrochemical-Electrochemical Dual-Mode Biosensor: Enabling Amplification-Free Detection of miRNA-133a in Acute Myocardial Infarction.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c06761
Abstract: Acute myocardial infarction (AMI) is a leading cause of death and disability worldwide. MicroRNA-133a (miRNA-133a) serves as a valuable biomarker for AMI, offering specificity and stability for an early diagnosis. However, existing miRNA biosensors faced… read more here.
Keywords: detection; myocardial infarction; amplification free; amplification ... See more keywords
CRISPR/Cas12a-Based Dynamic Light Scattering Assay for Nucleic Acid Amplification-Free and Ultrasensitive Detection of Listeria monocytogenes.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.5c04740
Abstract: Ultrasensitive signal transduction systems are essential for developing rapid, robust, and sensitive nucleic acid tests using the amplification-free CRISPR-Cas12a system. In this study, we introduce a novel platform termed CRISPR-DART (dynamic light scattering Assisted Rapid… read more here.
Keywords: nucleic acid; light scattering; crispr cas12a; amplification free ... See more keywords
An Amplification-Free Digital Assay Based on Primer Exchange Reaction-Mediated Botryoidal-Like Fluorescent Polystyrene Dots to Detect Multiple Pathogenic Bacteria.
Sign Up to like & getrecommendations! Published in 2024 at "ACS nano"
DOI: 10.1021/acsnano.4c09069
Abstract: Multiple and ultrasensitive detection of pathogenic bacteria is critical but remains a challenge. Here, we introduce a digital assay for multiplexed and target DNA amplification-free detection of pathogenic bacteria using botryoidal-like fluorescent polystyrene dots (PS-dots),… read more here.
Keywords: reaction; amplification free; botryoidal like; digital assay ... See more keywords
Amplification-Free, High-Throughput Nanoplasmonic Quantification of Circulating MicroRNAs in Unprocessed Plasma Microsamples for Earlier Pancreatic Cancer Detection.
Sign Up to like & getrecommendations! Published in 2023 at "ACS sensors"
DOI: 10.1021/acssensors.2c02105
Abstract: Pancreatic ductal adenocarcinoma (PDAC) is a deadly malignancy that is often detected at an advanced stage. Earlier diagnosis of PDAC is key to reducing mortality. Circulating biomarkers such as microRNAs are gaining interest, but existing… read more here.
Keywords: sensitivity; plasma; detection; amplification free ... See more keywords
Ultraspecific and Amplification-Free Quantification of Mutant DNA by Single-Molecule Kinetic Fingerprinting.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b06685
Abstract: Conventional techniques for detecting rare DNA sequences require many cycles of PCR amplification for high sensitivity and specificity, potentially introducing significant biases and errors. While amplification-free methods exist, they rarely achieve the ability to detect… read more here.
Keywords: molecule; single molecule; kinetic fingerprinting; dna ... See more keywords
Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc05607f
Abstract: Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence… read more here.
Keywords: flow assays; assays amplification; amplification free; oligonucleotide templated ... See more keywords