Detailed analysis of HTT repeat elements in human blood using targeted amplification‐free long‐read sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Human Mutation"
DOI: 10.1002/humu.23580
Abstract: Amplification of DNA is required as a mandatory step during library preparation in most targeted sequencing protocols. This can be a critical limitation when targeting regions that are highly repetitive or with extreme guanine–cytosine (GC)… read more here.
Keywords: repeat elements; analysis; repeat; amplification free ... See more keywords
Amplification-free detection of microRNAs via a rapid microarray-based sandwich assay
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0298-6
Abstract: The detection and profiling of microRNAs are of great interest in disease diagnosis and prognosis. In this paper, we present a method for the rapid amplification-free detection of microRNAs from total RNA samples. In a… read more here.
Keywords: detection; sandwich assay; free detection; amplification free ... See more keywords
Nucleic acid amplification-free detection of DNA and RNA at ultralow concentration.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2021.07.022
Abstract: The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10-18 M) concentration and lower is reviewed. These low concentrations correspond roughly to the most clinically desirable detection range… read more here.
Keywords: detection; free detection; amplification free; acid amplification ... See more keywords
Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02283
Abstract: CRISPR/Cas-based systems are highly attractive for developing next-generation diagnostic technologies because of their intrinsic merits such as simplicity, sensitivity, and specificity. However, currently, nucleic acid amplification procedures are still needed to achieve attomolar sensitivity in… read more here.
Keywords: dna; one step; amplification; single microbead ... See more keywords
Amplification-Free, High-Throughput Nanoplasmonic Quantification of Circulating MicroRNAs in Unprocessed Plasma Microsamples for Earlier Pancreatic Cancer Detection.
Sign Up to like & getrecommendations! Published in 2023 at "ACS sensors"
DOI: 10.1021/acssensors.2c02105
Abstract: Pancreatic ductal adenocarcinoma (PDAC) is a deadly malignancy that is often detected at an advanced stage. Earlier diagnosis of PDAC is key to reducing mortality. Circulating biomarkers such as microRNAs are gaining interest, but existing… read more here.
Keywords: sensitivity; plasma; detection; amplification free ... See more keywords
Ultraspecific and Amplification-Free Quantification of Mutant DNA by Single-Molecule Kinetic Fingerprinting.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b06685
Abstract: Conventional techniques for detecting rare DNA sequences require many cycles of PCR amplification for high sensitivity and specificity, potentially introducing significant biases and errors. While amplification-free methods exist, they rarely achieve the ability to detect… read more here.
Keywords: molecule; single molecule; kinetic fingerprinting; dna ... See more keywords
Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc05607f
Abstract: Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence… read more here.
Keywords: flow assays; assays amplification; amplification free; oligonucleotide templated ... See more keywords
An amplification-free colorimetric test for sensitive DNA detection based on the capturing of gold nanoparticle clusters.
Sign Up to like & getrecommendations! Published in 2020 at "Nanoscale"
DOI: 10.1039/d0nr03517c
Abstract: PCR-free or amplification-free strategies for DNA detection provide an interesting alternative to classical molecular biology techniques, opening new possibilities for on-site diagnostics. In this framework, we present herein an amplification-free colorimetric test for DNA detection,… read more here.
Keywords: colorimetric test; free colorimetric; detection; amplification free ... See more keywords
An amplification-free ultra-sensitive electrochemical CRISPR/Cas biosensor for drug-resistant bacteria detection
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Science"
DOI: 10.1039/d1sc02197d
Abstract: Continued development of high-performance and cost-effective in vitro diagnostic tools is vital for improving infectious disease treatment and transmission control. For nucleic acid diagnostics, moving beyond enzyme-mediated amplification assays will be critical in reducing the… read more here.
Keywords: detection; crispr cas; amplification free; cas biosensor ... See more keywords
Genome-wide integration site detection using Cas9 enriched amplification-free long-range sequencing
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1152
Abstract: Abstract The gene and cell therapy fields are advancing rapidly, with a potential to treat and cure a wide range of diseases, and lentivirus-based gene transfer agents are the vector of choice for many investigators.… read more here.
Keywords: integration; range; amplification free; integration site ... See more keywords