Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
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DOI: 10.1186/s13148-017-0328-4
Abstract: BackgroundDetermining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status… read more here.
Keywords: methylation; pcr amplification; amplification methylated; pcr ... See more keywords