32P-Postlabeling Analysis of DNA Adducts.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0223-2_16
Abstract: 32P-Postlabeling analysis is an ultra-sensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, and other DNA lesions resulting from… read more here.
Keywords: analysis dna; dna; 32p postlabeling; dna adducts ... See more keywords
Data Analysis of DNA Methylation Epigenome-Wide Association Studies (EWAS): A Guide to the Principles of Best Practice.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2140-0_2
Abstract: Array-based EWAS have become an increasingly popular technique to identify population epigenetic effects, particularly in humans. With the arrival of nonhuman species arrays, such as the mouse, this is likely to become an even more… read more here.
Keywords: analysis; analysis dna; methylation; dna methylation ... See more keywords
Analysis of DNA Hydroxymethylation Using Colorimetric Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4899-7708-3_8
Abstract: Hydroxymethylcytosine (hmC or 5-hmC) is a nitrogen base occurring as a result of cytosine methylation followed by replacing a methyl group with a hydroxyl group through active oxidation. 5-hmC is considered to be one of… read more here.
Keywords: analysis dna; hydroxymethylation using; dna; using colorimetric ... See more keywords
Determining the impact of unknown individuals in criminality using network analysis of DNA matches.
Sign Up to like & getrecommendations! Published in 2021 at "Forensic science international"
DOI: 10.1016/j.forsciint.2021.111142
Abstract: Criminal offenders missing from police files limit the capacity to reconstruct criminal networks for criminological research and operational purposes. Recent studies show that forensic DNA databanks offer potential to address this problem, through large-scale analysis… read more here.
Keywords: dna; analysis dna; dna matches; network ... See more keywords
DNA framework-supported electrochemical analysis of DNA methylation for prostate cancers.
Sign Up to like & getrecommendations! Published in 2020 at "Nano letters"
DOI: 10.1021/acs.nanolett.0c01898
Abstract: Epigenetic alterations hold great promise as biomarkers for early-stage cancer diagnosis. Nevertheless, direct identification of rare methylated DNA in the genome remains challenging. Here we report an ultrasensitive framework nucleic acid-based electrochemical sensor for quantitative… read more here.
Keywords: analysis dna; methylation; dna; framework ... See more keywords
mHapTk: a comprehensive toolkit for the analysis of DNA methylation haplotypes
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac650
Abstract: SUMMARY Bisulfite sequencing (BS-seq) remains the gold standard technique to detect DNA methylation profiles at single-nucleotide resolution. The DNA methylation status of CpG sites on the same fragment represents a discrete methylation haplotype (mHap). The… read more here.
Keywords: methylation; toolkit analysis; dna methylation; mhaptk comprehensive ... See more keywords
High-throughput quantitative binding analysis of DNA aptamers using exonucleases
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1210
Abstract: Abstract Aptamers are nucleic acid bioreceptors that have been used in various applications including medical diagnostics and as therapeutic agents. Identifying the most optimal aptamer for a particular application is very challenging. Here, we for… read more here.
Keywords: high throughput; binding analysis; quantitative binding; analysis dna ... See more keywords
Analysis of DNA Methylation in Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top094821
Abstract: Methylation of DNA, the most experimentally accessible epigenetic alteration of eukaryotic cells, has generated an extensive literature and an abundance of analytical tools. The term "methylome" (referring to the complete set of cytosine modifications in… read more here.
Keywords: analysis dna; methylation; dna; methylation mammalian ... See more keywords
Analysis of DNA methylation and microRNA expression in NUT (nuclear protein in testis) midline carcinoma of the sinonasal tract: a clinicopathological, immunohistochemical and molecular genetic study.
Sign Up to like & getrecommendations! Published in 2018 at "Neoplasma"
DOI: 10.4149/neo_2018_161122n581
Abstract: The aim of this study was a detailed clinicopathological investigation of sinonasal NUT midline carcinoma (NMC), including analysis of DNA methylation and microRNA (miRNA) expression. Three (5%) cases of NMC were detected among 56 sinonasal… read more here.
Keywords: analysis dna; methylation; midline carcinoma; expression ... See more keywords