Putting a New Spin of G-Quadruplex Structure and Binding by Analytical Ultracentrifugation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9666-7_5
Abstract: Analytical ultracentrifugation is a powerful biophysical tool that provides information about G-quadruplex structure, stability, and binding reactivity. This chapter provides a simplified explanation of the method, along with examples of how it can be used… read more here.
Keywords: analytical ultracentrifugation; quadruplex structure; putting new; new spin ... See more keywords
Measurement of length distribution of beta-lactoglobulin fibrils by multiwavelength analytical ultracentrifugation
Sign Up to like & getrecommendations! Published in 2020 at "European Biophysics Journal"
DOI: 10.1007/s00249-020-01421-4
Abstract: The whey protein beta-lactoglobulin is the building block of amyloid fibrils which exhibit a great potential in various applications. These include stabilization of gels or emulsions. During biotechnological processing, high shear forces lead to fragmentation… read more here.
Keywords: sedimentation; analytical ultracentrifugation; beta lactoglobulin; distribution ... See more keywords
Probing the oligomeric re-assembling of bacterial fimbriae in vitro: a small-angle X-ray scattering and analytical ultracentrifugation study
Sign Up to like & getrecommendations! Published in 2021 at "European Biophysics Journal"
DOI: 10.1007/s00249-021-01543-3
Abstract: Capsular antigen fragment 1 (Caf1) is an oligomeric protein consisting of 15 kDa monomeric subunits that are non-covalently linked through exceptionally strong and kinetically inert interactions into a linear polymer chain. It has been shown… read more here.
Keywords: small angle; caf1; polymer; angle ray ... See more keywords
Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Reviews"
DOI: 10.1007/s12551-017-0374-3
Abstract: Sedimentation velocity analytical ultracentrifugation (SV-AUC) coupled with direct computational fitting of the observed concentration profiles (sedimentating boundary) have been developed and widely used for the characterization of macromolecules and nanoparticles in solution. In particular, size… read more here.
Keywords: therapeutic antibodies; velocity analytical; sedimentation velocity; analytical ultracentrifugation ... See more keywords
Quantitation of soluble aggregates by sedimentation velocity analytical ultracentrifugation using an optical alignment system - aspects of method validation.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113837
Abstract: Sedimentation velocity analytical ultracentrifugation (SV-AUC) is routinely used for quantitation of soluble aggregates as an orthogonal technique to size-exclusion chromatography (SEC). SV-AUC presents many advantages over the SEC, yet lower precision of aggregate quantitation by… read more here.
Keywords: velocity analytical; system; quantitation soluble; quantitation ... See more keywords
Reconstitution of full-length human caveolin-1 into phospholipid bicelles: Validation by analytical ultracentrifugation.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical chemistry"
DOI: 10.1016/j.bpc.2020.106339
Abstract: A significant hurdle in obtaining biophysical information on membrane proteins is developing a successful strategy for their reconstitution into a suitable membrane mimic. In particular, utilization of the more 'native-like' membrane mimics such as bicelles… read more here.
Keywords: full length; reconstitution; caveolin; analytical ultracentrifugation ... See more keywords
Identification of nanomaterials: A validation report of two laboratories using analytical ultracentrifugation with fixed and ramped speed options
Sign Up to like & getrecommendations! Published in 2018 at "NanoImpact"
DOI: 10.1016/j.impact.2017.12.005
Abstract: Abstract The identification of nanomaterials for regulatory purposes in Europe requires a statistically significant comparison of the number metrics median diameter to the 100 nm criterion. Methods should be robust against minor imperfections of the implementation,… read more here.
Keywords: identification nanomaterials; validation report; ultracentrifugation fixed; identification ... See more keywords
Determination of 2D Particle Size Distributions in Plasmonic Nanoparticle Colloids via Analytical Ultracentrifugation: Application to Gold Bipyramids.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.2c12257
Abstract: Multidimensional particle properties determine the product properties in numerous advanced applications. Accurate and statistically meaningful measurements of complex particles and their multidimensional distributions are highly challenging but strongly needed. 2D particle size distributions of plasmonic… read more here.
Keywords: particle size; distributions plasmonic; microscopy; size distributions ... See more keywords
Characterisation of mass distributions of solvent-fractionated lignins using analytical ultracentrifugation and size exclusion chromatography methods
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-93424-0
Abstract: Lignins are valuable renewable resources for the potential production of a large array of biofuels, aromatic chemicals and biopolymers. Yet native and industrial lignins are complex, highly branched and heterogenous macromolecules, properties that have to… read more here.
Keywords: mass; exclusion chromatography; lignins using; analytical ultracentrifugation ... See more keywords
Integral approach to biomacromolecular structure by analytical-ultracentrifugation and small-angle scattering
Sign Up to like & getrecommendations! Published in 2020 at "Communications Biology"
DOI: 10.1038/s42003-020-1011-4
Abstract: Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make… read more here.
Keywords: analytical ultracentrifugation; small angle; angle scattering; sas ... See more keywords
ICCD camera technology with constant illumination source and possibilities for application in multiwavelength analytical ultracentrifugation
Sign Up to like & getrecommendations! Published in 2018 at "RSC Advances"
DOI: 10.1039/c8ra08752k
Abstract: A new concept for multiwavelength detection in analytical ultracentrifugation is demonstrated, based on the technology of an Andor iStar intensified CCD camera and constant illumination source. The camera is coupled to an Andor Shamrock spectrograph… read more here.
Keywords: constant illumination; illumination; illumination source; camera ... See more keywords