Unveiling Highly Active and Stable l-Glutaminase through Ancestral Sequence Reconstruction and Turnover Number Prediction.
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DOI: 10.1021/acs.jafc.4c11502
Abstract: In our study, we employed ancestral sequence reconstruction and DLKcat analysis to engineer l-glutaminases with enhanced activity and thermal stability, using Bacillus subtilis 168 l-glutaminase (YbgJ) as the template. We identified two ancestral l-glutaminases, Anc165… read more here.
Keywords: anc165; sequence reconstruction; ancestral sequence; activity ... See more keywords