Engineering the Thermostability of a d-Carbamoylase Based on Ancestral Sequence Reconstruction for the Efficient Synthesis of d-Tryptophan.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c07781
Abstract: Employing ancestral sequence reconstruction and consensus sequence analysis, the thermostability of a novel d-carbamoylase derived from Nitratireductor indicus (NiHyuC) was engineered through greedy-oriented iterative combinatorial mutagenesis. A mutant S202P/E208D/R277L (M4Th3) was obtained with significantly elevated… read more here.
Keywords: ancestral sequence; carbamoylase; sequence; thermostability ... See more keywords
Deciphering the evolution of flavin-dependent monooxygenase stereoselectivity using ancestral sequence reconstruction.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2218248120
Abstract: Controlling the selectivity of a reaction is critical for target-oriented synthesis. Accessing complementary selectivity profiles enables divergent synthetic strategies, but is challenging to achieve in biocatalytic reactions given enzymes' innate preferences of a single selectivity.… read more here.
Keywords: flavin dependent; ancestral sequence; reaction; stereoselectivity ... See more keywords
Consequences of Substitution Model Selection on Protein Ancestral Sequence Reconstruction
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology and Evolution"
DOI: 10.1093/molbev/msac144
Abstract: Abstract The selection of the best-fitting substitution model of molecular evolution is a traditional step for phylogenetic inferences, including ancestral sequence reconstruction (ASR). However, a few recent studies suggested that applying this procedure does not… read more here.
Keywords: substitution; substitution model; ancestral sequence; reconstruction ... See more keywords
ARPIP: Ancestral sequence Reconstruction with insertions and deletions under the Poisson Indel Process.
Sign Up to like & getrecommendations! Published in 2022 at "Systematic biology"
DOI: 10.1093/sysbio/syac050
Abstract: Modern phylogenetic methods allow inference of ancestral molecular sequences given an alignment and phylogeny relating present day sequences. This provides insight into the evolutionary history of molecules, helping to understand gene function and to study… read more here.
Keywords: insertions deletions; reconstruction insertions; ancestral sequence; poisson indel ... See more keywords
Engineering indel and substitution variants of diverse and ancient enzymes using Graphical Representation of Ancestral Sequence Predictions (GRASP)
Sign Up to like & getrecommendations! Published in 2022 at "PLOS Computational Biology"
DOI: 10.1101/2019.12.30.891457
Abstract: Ancestral sequence reconstruction is a technique that is gaining widespread use in molecular evolution studies and protein engineering. Accurate reconstruction requires the ability to handle appropriately large numbers of sequences, as well as insertion and… read more here.
Keywords: representation ancestral; indel; ancestral sequence; sequence ... See more keywords
Corrigendum: Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.650373
Abstract: [This corrects the article DOI: 10.3389/fmicb.2020.625837.]. read more here.
Keywords: motb proton; proton motile; reconstructions motb; sequence reconstructions ... See more keywords
Preparation and Characterization of an Ancient Aminopeptidase Obtained from Ancestral Sequence Reconstruction for L-Carnosine Synthesis
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27196620
Abstract: As a biologically active peptide, L-carnosine has been widely used in the pharmaceutical, cosmetic and health care industries due to its various physiological properties. However, relatively little research is available regarding L-carnosine’s enzymatic synthesis function.… read more here.
Keywords: carnosine; ancestral sequence; carnosine synthesis; synthesis ... See more keywords