Supercritical angle fluorescence for enhanced axial sectioning in STED microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.03.027
Abstract: We demonstrate subwavelength axial sectioning on biological samples with a stimulated emission depletion (STED) microscope combined with supercritical angle fluorescence (SAF) detection. SAF imaging is a powerful technique for imaging the membrane of the cell… read more here.
Keywords: angle; axial sectioning; microscopy; angle fluorescence ... See more keywords
A Novel Interference-Based Supercritical Angle Fluorescence Biosensor.
Sign Up to like & getrecommendations! Published in 2025 at "ACS sensors"
DOI: 10.1021/acssensors.5c02258
Abstract: Supercritical angle fluorescence (SAF) is a near-field collection technique used to create surface-sensitive optical transducers. While SAF biosensors require simpler excitation optics compared with total internal reflection fluorescence (TIRF)-based systems, they rely on complex fluorescence… read more here.
Keywords: novel interference; fluorescence; angle fluorescence; supercritical angle ... See more keywords