A high‐resolution (1.2 Å) crystal structure of the anti‐CRISPR protein AcrIF9
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12986
Abstract: Prokaryotic adaptive immunity by CRISPR‐Cas systems, which confer resistance to foreign genetic elements, has been used by bacteria to combat viruses. To cope, viruses evolved multiple anti‐CRISPR proteins, which can inhibit system function through various… read more here.
Keywords: crystal structure; high resolution; resolution crystal; anti crispr ... See more keywords
Crystal structure of the anti‐CRISPR, AcrIIC4
Sign Up to like & getrecommendations! Published in 2021 at "Protein Science"
DOI: 10.1002/pro.4214
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPRs)‐CRISPR‐associated protein systems are bacterial and archaeal defense mechanisms against invading elements such as phages and viruses. To overcome these defense systems, phages and viruses have developed inhibitors called… read more here.
Keywords: crystal structure; inhibitory mechanisms; crispr; anti crispr ... See more keywords
A 1.3 Å high-resolution crystal structure of an anti-CRISPR protein, AcrI E2.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.09.067
Abstract: As a result of bacterial infection with viruses, bacteria have developed CRISPR-Cas as an adaptive immune system, which allows them to destroy the viral genetic material introduced via infection. However, viruses have also evolved to… read more here.
Keywords: crystal structure; high resolution; crispr; resolution crystal ... See more keywords
A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein.
Sign Up to like & getrecommendations! Published in 2018 at "Structure"
DOI: 10.1016/j.str.2018.04.008
Abstract: Virus capsids are protein shells that protect the viral genome from environmental assaults, while maintaining the high internal pressure of the tightly packaged genome. To elucidate how capsids maintain stability under harsh conditions, we investigated… read more here.
Keywords: protein; crispr protein; hyperthermophilic phage; decoration ... See more keywords
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.
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DOI: 10.1016/j.ymeth.2019.05.014
Abstract: The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can… read more here.
Keywords: transcription translation; free transcription; cell free; translation system ... See more keywords
Anti-CRISPRdb v2.2: an online repository of anti-CRISPR proteins including information on inhibitory mechanisms, activities and neighbors of curated anti-CRISPR proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Database : the journal of biological databases and curation"
DOI: 10.1093/database/baac010
Abstract: We previously released the Anti-CRISPRdb database hosting anti-CRISPR proteins (Acrs) and associated information. Since then, the number of known Acr families, types, structures and inhibitory activities has accumulated over time, and Acr neighbors can be… read more here.
Keywords: crispr proteins; crispr; information; anti crisprdb ... See more keywords
Phages, anti-CRISPR proteins, and drug-resistant bacteria: what do we know about this triad?
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens and disease"
DOI: 10.1093/femspd/ftac039
Abstract: Phages are viruses that infect bacteria, relying on their genetic machinery to replicate. To survive the constant attack of phages, bacteria have developed diverse defense strategies to act against them. Nevertheless, phages rapidly co-evolve to… read more here.
Keywords: proteins drug; crispr proteins; anti crispr; phages anti ... See more keywords
Mobile element warfare via CRISPR and anti-CRISPR in Pseudomonas aeruginosa
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab006
Abstract: Abstract Bacteria deploy multiple defenses to prevent mobile genetic element (MGEs) invasion. CRISPR–Cas immune systems use RNA-guided nucleases to target MGEs, which counter with anti-CRISPR (Acr) proteins. Our understanding of the biology and co-evolutionary dynamics… read more here.
Keywords: pseudomonas aeruginosa; crispr; crispr cas; anti crispr ... See more keywords
Molecular basis of anti-CRISPR operon repression by Aca10
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DOI: 10.1093/nar/gkac656
Abstract: Abstract CRISPR-Cas systems are bacterial defense systems for fighting against invaders such as bacteriophages and mobile genetic elements. To escape destruction by these bacterial immune systems, phages have co-evolved multiple anti-CRISPR (Acr) proteins, which inhibit… read more here.
Keywords: operon; crispr; anti crispr; aca10 ... See more keywords
Widespread repression of anti-CRISPR production by anti-CRISPR-associated proteins
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac674
Abstract: Abstract Many bacteria use CRISPR-Cas systems to defend against invasive mobile genetic elements (MGEs). In response, MGEs have developed strategies to resist CRISPR-Cas, including the use of anti-CRISPR (Acr) proteins. Known acr genes may be… read more here.
Keywords: widespread repression; crispr; anti crispr; repression anti ... See more keywords
Molecular basis of dual anti-CRISPR and auto-regulatory functions of AcrIF24
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac880
Abstract: Abstract CRISPR-Cas systems are adaptive immune systems in bacteria and archaea that provide resistance against phages and other mobile genetic elements. To fight against CRISPR-Cas systems, phages and archaeal viruses encode anti-CRISPR (Acr) proteins that… read more here.
Keywords: acr aca; crispr cas; anti crispr; acr ... See more keywords