Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6759-9_8
Abstract: Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant primary antibodies. Several techniques can… read more here.
Keywords: host species; raised host; two color; hapten anti ... See more keywords
Antibodies raised against aldehyde-fixed antigens improve sensitivity for postembedding electron microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2019.01.015
Abstract: BACKGROUND Antibodies are one of the most important tools in biological research. High specificity and sensitivity of antibodies are crucial to obtain reliable results. Tissue fixed with glutaraldehyde (GA) is commonly used in electron microscopical… read more here.
Keywords: microscopy; improve sensitivity; antibodies raised; fixed antigens ... See more keywords
Pseudomonas aeruginosa keratitis: passive immunotherapy with antibodies raised against divalent flagellin
Sign Up to like & getrecommendations! Published in 2019 at "Iranian Journal of Basic Medical Sciences"
DOI: 10.22038/ijbms.2018.31499.7643
Abstract: Objective(s): Pseudomonas aeruginosa infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of… read more here.
Keywords: pseudomonas aeruginosa; antibodies raised; aeruginosa keratitis; divalent flagellin ... See more keywords