Creation of Recombinant Antibodies: Using 5'-RACE to Amplify Immunoglobulin Sequences.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot093856
Abstract: Modern molecular biology techniques have been applied to the production of therapeutic antibodies. Nonetheless, recombinant antibodies remain the exception rather than the rule for the antibodies used in most research and diagnostic applications. The lack… read more here.
Keywords: creation recombinant; antibodies using; race; gene ... See more keywords
Labeling Antibodies Using a Maleimido Dye.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099291
Abstract: Fluorophore-maleimide derivatives are effective for labeling sulfhydryl-containing molecules. Maleimide groups react with free thiols at pH 6.5-7.5 forming a covalent bond. Reducing agents should be avoided during the conjugation step. This protocol uses the cross-linker… read more here.
Keywords: antibodies using; using maleimido; labeling antibodies; maleimido dye ... See more keywords
Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein
Sign Up to like & getrecommendations! Published in 2021 at "Virology Journal"
DOI: 10.1186/s12985-021-01490-7
Abstract: Background SARS-CoV-2 is a novel coronavirus that emerged in 2019 and is now classified in the genus Coronavirus with closely related SARS-CoV. SARS-CoV-2 is highly pathogenic in humans and is classified as a biosafety level… read more here.
Keywords: neutralizing antibodies; evaluation sars; cov neutralizing; antibodies using ... See more keywords