An epitope‐directed antibody affinity maturation system utilizing mammalian cell survival as readout
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26965
Abstract: Upon developing therapeutically potent antibodies, there are significant requirements, such as increasing their affinity, regulating their epitope, and using native target antigens. Many antibody selection systems, such as a phage display method, have been developed,… read more here.
Keywords: cell; system; affinity; antibody affinity ... See more keywords
Determination of O:4 antigen‐antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium
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DOI: 10.1093/femsle/fnx062
Abstract: Abstract Salmonella enterica serovar Typhimurium (S. Typhimurium) has two serological variants: one that expresses the O:5 antigen (1,4,5,12:i:1,2) and one that lacks O:5 antigen (1,4,12:i:1,2). For serotyping, S. Typhimurium is agglutinated by diagnostic O:4 antigen… read more here.
Keywords: typhimurium; antigen; antigen antibody; antibody affinity ... See more keywords
SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz050
Abstract: Abstract Mutagenesis libraries are essential for combinatorial protein engineering. Despite improvements in gene synthesis and directed mutagenesis, current methodologies still have limitations regarding the synthesis of complete antibody single-chain variable fragment (scFv) genes and simultaneous… read more here.
Keywords: mutagenesis; antibody affinity; affinity maturation; solid phase ... See more keywords
Enhancers Improve the AID-Induced Hypermutation in Episomal Vector for Antibody Affinity Maturation in Mammalian Cell Display
Sign Up to like & getrecommendations! Published in 2018 at "Antibodies"
DOI: 10.3390/antib7040042
Abstract: The induction of somatic hypermutation (SHM) in various cell lines by activation-induced cytidine deaminase (AID) has been used in protein-directed selection, especially in antibody affinity maturation. Several antibody affinity maturation systems based on mammalian cells… read more here.
Keywords: antibody; antibody affinity; aid; hypermutation ... See more keywords