The Allosteric Effect in Antibody-Antigen Recognition.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1154-8_11
Abstract: We studied the molecular details of the recognition of antigens by the variable domain of their cognate antibodies in as well as those elicited by the constant domains, which do not directly interact with antigens.… read more here.
Keywords: effect antibody; allosteric effect; antigen recognition; recognition ... See more keywords
Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8648-4_22
Abstract: Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a diverse range of protein-protein interactions and is considered almost de rigueur for characterizing antibody-antigen interactions. The technique obviates the need to… read more here.
Keywords: plasmon resonance; antibody; antibody antigen; surface plasmon ... See more keywords
An Approach to Bioactivity Assessment for Critical Quality Attribute Identification Based on Antibody-Antigen Complex Structure.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2020.12.027
Abstract: Identification of critical quality attributes (CQAs) is an important step for development of biopharmaceuticals with intended performance. An accurate CQA assessment is needed to ensure product quality and focusing on development efforts where control is… read more here.
Keywords: bioactivity; quality; bioactivity assessment; antigen complex ... See more keywords
Complementary Structural Information for Antibody-Antigen Complexes from Hydrogen-Deuterium Exchange and Covalent Labeling Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00108
Abstract: Characterizing antibody-antigen interactions is necessary for properly developing therapeutic antibodies, understanding their mechanisms of action, and patenting new drug molecules. Here, we demonstrate that hydrogen-deuterium exchange (HDX) mass spectrometry (MS) measurements together with diethylpyrocarbonate (DEPC)… read more here.
Keywords: information; antibody antigen; antigen; mass spectrometry ... See more keywords
Assessment of software methods for estimating protein-protein relative binding affinities
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DOI: 10.1371/journal.pone.0240573
Abstract: A growing number of computational tools have been developed to accurately and rapidly predict the impact of amino acid mutations on protein-protein relative binding affinities. Such tools have many applications, for example, designing new drugs… read more here.
Keywords: binding affinities; antibody antigen; relative binding;
AbAgIntPre: A deep learning method for predicting antibody-antigen interactions based on sequence information
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1053617
Abstract: Introduction Antibody-mediated immunity is an essential part of the immune system in vertebrates. The ability to specifically bind to antigens allows antibodies to be widely used in the therapy of cancers and other critical diseases.… read more here.
Keywords: abagintpre deep; antigen interactions; antibody antigen; deep learning ... See more keywords
Antibody-Antigen Binding Interface Analysis in the Big Data Era
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.945808
Abstract: Antibodies have become the Swiss Army tool for molecular biology and nanotechnology. Their outstanding ability to specifically recognise molecular antigens allows their use in many different applications from medicine to the industry. Moreover, the improvement… read more here.
Keywords: antibody antigen; analysis; biology; antigen binding ... See more keywords
Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues
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DOI: 10.3390/bios12080560
Abstract: To produce a sensitive monoclonal antibody (mAb) for the simultaneous detection of carbofuran, benfuracarb, carbosulfan and 3-hydroxy-carbofuran, 2,3-dihydro-2,2-dimethyl-7-benzofuranmethanamine (DDB) was conjugated to bovine serum albumin (BSA) to prepare the immunogen DDB-BSA and mice were immunized.… read more here.
Keywords: carbofuran; development; antibody antigen; antigen pairs ... See more keywords