Specific epitopes form extensive hydrogen-bonding networks to ensure efficient antibody binding of SARS-CoV-2: Implications for advanced antibody design
Sign Up to like & getrecommendations! Published in 2021 at "Computational and Structural Biotechnology Journal"
DOI: 10.1016/j.csbj.2021.03.021
Abstract: Neutralizing antibody targeting to the SARS-CoV-2 could provide powerful therapies. A neutralizing antibody CC12.1 which was found in SARS-CoV-2 patient samples provides potential protection from disease. The aim of molecular dynamics simulations is to identify… read more here.
Keywords: antibody; binding sars; key epitopes; rbd ... See more keywords
Polyvalent immunoglobulin binding is an obstacle to accurate measurement of specific antibodies with ELISA despite inclusion of blocking agents.
Sign Up to like & getrecommendations! Published in 2017 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2017.09.016
Abstract: Specific antibody concentrations are frequently measured in serum (and plasma and intravenous immunoglobulin) samples by enzyme-linked immunosorbent assay (ELISA). The standard negative control involves incubation of buffer alone on antigen-coated wells. The immunoreactivity that develops… read more here.
Keywords: immunoglobulin; blocking agents; specific antibody; antibody ... See more keywords
Quantifying Antibody Binding Kinetics on Fixed Cells and Tissues via Fluorescence Lifetime Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01076
Abstract: We present a method for monitoring spatially localized antigen-antibody binding events on physiologically relevant substrates (cell and tissue sections) using fluorescence lifetime imaging. Specifically, we use the difference between the fluorescence decay times of fluorescently… read more here.
Keywords: binding kinetics; fluorescence lifetime; antibody binding; lifetime imaging ... See more keywords
Residue-Level Characterization of Antibody Binding Epitopes Using Carbene Chemical Footprinting.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03091
Abstract: Characterization of antibody binding epitopes is an important factor in therapeutic drug discovery, as the binding site determines and drives antibody pharmacology and pharmacokinetics. Here, we present a novel application of carbene chemical footprinting with… read more here.
Keywords: characterization antibody; binding epitopes; level; antibody binding ... See more keywords
Characterization of Phosphorylation-Dependent Antibody Binding to Cancer-Mutated Linkers of C2H2 Zinc Finger Proteins by Intact Transition Epitope Mapping-Thermodynamic Weak-Force Order Analysis.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00244
Abstract: With Intact Transition Epitope Mapping-Thermodynamic Weak-force Order (ITEM-TWO) analysis in combination with molecular modeling, the phosphorylation-dependent molecular recognition motif of the anti-HpTGEKP antibody has been investigated with binary and ternary component mixtures consisting of antibody… read more here.
Keywords: antibody binding; analysis; intact transition; transition epitope ... See more keywords
A New Antibody Binding Test for Potency Assessment of the Human Rabies Vaccine.
Sign Up to like & getrecommendations! Published in 2023 at "Viral immunology"
DOI: 10.1089/vim.2022.0161
Abstract: This study established a new protocol of the antibody binding test to evaluate the potency of the rabies vaccine containing the final bulk and the product. The principle of this experiment is to combine rabies… read more here.
Keywords: rabies vaccine; binding test; potency; antibody binding ... See more keywords
Inhibiting N-glycan processing increases the antibody binding affinity and effector function of human natural killer cells.
Sign Up to like & getrecommendations! Published in 2023 at "Immunology"
DOI: 10.1111/imm.13662
Abstract: Novel approaches are required to improve the efficacy of immunotherapies and increase the proportion of patients who experience a benefit. Antibody-dependent cell-mediated cytotoxicity (ADCC) contributes to the efficacy of many monoclonal antibodies therapies. Natural killer… read more here.
Keywords: cell; antibody binding; cd16a; glycan processing ... See more keywords
Downregulation of Gabarapl1 significantly attenuates antibody binding to porcine aortic endothelial cells
Sign Up to like & getrecommendations! Published in 2019 at "Xenotransplantation"
DOI: 10.1111/xen.12537
Abstract: After hyperacute rejection in pig‐to‐primate xenotransplantation had been overcome by the introduction of α1,3‐galactosyltransferase gene‐knockout (GTKO) pigs, acute and chronic antibody‐mediated rejection became one of the major barriers to long‐term graft survival. This was associated… read more here.
Keywords: porcine aortic; antibody; endothelial cells; antibody binding ... See more keywords
Serum Antibody Binding and Cytotoxicity to Pig Cells in Chinese Subjects: Relevance to Clinical Renal Xenotransplantation
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.844632
Abstract: Kidney xenotransplantation is expected to contribute to resolving the shortage of kidneys from deceased human donors. Although progress in experimental life-supporting pig renal xenotransplantation has been encouraging, there are still issues to be considered before… read more here.
Keywords: pig; cmahko; antibody binding; gtko ... See more keywords
A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12061380
Abstract: Kynurenine is a tryptophan metabolite linked to several inflammatory processes including transplant failure, a significant challenge in transplant medicine. The detection of small molecules such as kynurenine, however, is often complex and time consuming. Herein,… read more here.
Keywords: kynurenine potential; transplant failure; potential biomarker; antibody binding ... See more keywords
The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23095049
Abstract: Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach… read more here.
Keywords: unspecific antibody; glucocorticoid receptor; anti glucocorticoid; antibody binding ... See more keywords