Phenomenon of Endothelial Antibody Capture: Principles and Potential for Locoregional Targeting of Hepatic Tumors
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DOI: 10.1002/hep.30072
Abstract: The systemic drug circulation represents a source of adverse effects during tumor targeting. We studied the binding efficacy of endothelium‐specific antibodies after a very short contact with an antigen target, along with assessing the intravascular… read more here.
Keywords: hepatic tumors; phenomenon endothelial; targeting hepatic; antibody ... See more keywords
Hybridoma Screening by Antibody Capture: Enzyme-Linked Detection (Indirect ELISA) in Polyvinyl Chloride Wells.
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DOI: 10.1101/pdb.prot103044
Abstract: In this antibody capture assay for hybridoma screening, the antigen is immobilized on a solid substrate (the surface of the wells in a polyvinyl chloride [PVC] microtiter plate), and antibodies in the hybridoma tissue culture… read more here.
Keywords: hybridoma screening; polyvinyl chloride; antibody; antibody capture ... See more keywords
Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Permeabilized Cells to Detect Intracellular Binding.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103085
Abstract: Flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal antibodies to internal cellular proteins, but the cells must be permeabilized before the hybridoma supernatants are applied. In using this… read more here.
Keywords: hybridoma screening; flow cytometry; screening antibody; cytometry ... See more keywords
Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence.
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DOI: 10.1101/pdb.prot103101
Abstract: Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a… read more here.
Keywords: capture detection; hybridoma screening; screening antibody; immunofluorescence ... See more keywords