Advances in the Production and Batch Reformatting of Phage Antibody Libraries
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biotechnology"
DOI: 10.1007/s12033-019-00207-0
Abstract: Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of highly… read more here.
Keywords: phage; antibody libraries; batch reformatting; advances production ... See more keywords
Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short reads.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad235
Abstract: Deep parallel sequencing (NGS) is a viable tool for monitoring scFv and Fab library dynamics in many antibody engineering high-throughput screening efforts. Although very useful, the commonly used Illumina NGS platform cannot handle the entire… read more here.
Keywords: full length; antibody libraries; homologous antibody; highly homologous ... See more keywords
Single-step colony assay with autoinduction of scFv expression for the screening of antibody libraries.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2018-0195
Abstract: We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv… read more here.
Keywords: antibody libraries; antibody; colony assay; expression ... See more keywords
Antibody Libraries as Tools to Discover Functional Antibodies and Receptor Pleiotropism
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22084123
Abstract: Most antibodies currently in use have been selected based on their binding affinity. However, nowadays, antibodies that can not only bind but can also alter the function of cell surface signaling components are increasingly sought… read more here.
Keywords: tools discover; functional antibodies; antibody libraries; antibody ... See more keywords