Using different proteolytic enzymes to digest antibody and its impact on stability of antibody mimetics.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2020.112933
Abstract: There are opportunities to formulate antibodies as solid-state depots for local therapy, which would minimise large systemic doses that are typically required. We have developed antibody mimetics known as Fab-PEG-Fab (FpF) that display similar binding… read more here.
Keywords: fpf; igg; using different; antibody ... See more keywords
Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use
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DOI: 10.1038/s41598-020-57713-4
Abstract: Monoclonal antibodies (mAbs) are attractive therapeutics for treating a wide range of human disorders, and bind to the antigen through their complementarity-determining regions (CDRs). Small stable proteins containing structurally retained CDRs are promising alternatives to… read more here.
Keywords: strategy; antibody mimetics; complementarity determining; create ... See more keywords
Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-02487-2
Abstract: Protein-based targeting reagents, such as antibodies and non-antibody scaffold proteins, are rapidly inactivated in the upper gastrointestinal (GI) tract. Hydrochloric acid in gastric juice denatures proteins and activates pepsin, concentrations of which reach 1 mg/mL in… read more here.
Keywords: pepsin; antibody; hydrochloric acid; antibody mimetics ... See more keywords