Improving the Developability of an Antigen Binding Fragment by Aspartate Substitutions.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00251
Abstract: Aggregation can be a major challenge in the development of antibody-based pharmaceuticals as it can compromise the quality of the product during bioprocessing, formulation, and drug administration. To avoid aggregation, developability assessment is often run… read more here.
Keywords: developability; aggregation; antigen binding; asp substitutions ... See more keywords
Site-Specific Fluorescent Labeling of Hemagglutinin-Specific Antigen Binding Fragment through Amine Chemistry Revealed by Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00330
Abstract: To capture the structure of assembled hemagglutinin (HA) nanoparticles at single-particle resolution, HA-specific antigen binding fragments (Fabs) were labeled by fluorescent (FLR) dyes as probes to highlight the HA trimers displayed on the assembled tetravalent… read more here.
Keywords: chemistry; specific antigen; antigen binding; site specific ... See more keywords
The role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-02756-3
Abstract: Antibody research has traditionally focused on heavy chains, often neglecting the important complementary role of light chains in antibody formation and secretion. In the light chain, the complementarity-determining region 3 (VL-CDR3) is specifically implicated in… read more here.
Keywords: framework region; antigen binding; recombinant production; antibody ... See more keywords
Repertoire-scale measures of antigen binding
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2203505119
Abstract: Antibodies and T-cell receptors (TCRs) are the fundamental building blocks of adaptive immunity. Repertoire-scale functionality derives from their epitope-binding properties, just as macroscopic properties like temperature derive from microscopic molecular properties. However, most approaches to… read more here.
Keywords: function; measures antigen; scale measures; binding properties ... See more keywords
One size does not fit all: navigating the multi-dimensional space to optimize T-cell engaging protein therapeutics
Sign Up to like & getrecommendations! Published in 2021 at "mAbs"
DOI: 10.1080/19420862.2020.1871171
Abstract: ABSTRACT T-cell engaging biologics is a class of novel and promising immune-oncology compounds that leverage the immune system to eradicate cancer. Here, we compared and contrasted a bispecific diabody-Fc format, which displays a relatively short… read more here.
Keywords: one size; antigen binding; cell engaging; size fit ... See more keywords
Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats
Sign Up to like & getrecommendations! Published in 2023 at "mAbs"
DOI: 10.1080/19420862.2023.2189432
Abstract: ABSTRACT Bispecific antibodies (bsAbs) enable dual binding of different antigens with potential synergistic targeting effects and innovative therapeutic possibilities. The formation of bsAbs is, however, often dependent on complex engineering strategies with a high risk… read more here.
Keywords: fusion; fusion single; single domain; antigen binding ... See more keywords
Characterization of a humanized PD-L1 blocking antibody with pH-dependent antigen binding in a humanized syngenic tumor model.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2017.35.7_suppl.81
Abstract: 81Background: Checkpoint inhibition is a promising approach for cancer treatment with durable responses and survival benefits. Currently, immune-checkpoint inhibitors are delivered at maximally tolerated doses, resulting in significant toxicities and cost burden. There is significant… read more here.
Keywords: tumor model; antigen binding; dependent antigen; antibody dependent ... See more keywords
Phage-display reveals interaction of lipocalin allergen Can f 1 with a peptide resembling the antigen binding region of a human γδT-cell receptor
Sign Up to like & getrecommendations! Published in 2020 at "Biological Chemistry"
DOI: 10.1515/hsz-2020-0185
Abstract: Abstract Although some progress has been achieved in understanding certain aspects of the allergenic mechanism of animal lipocalins, they still remain largely enigmatic. One possibility to unravel this property is to investigate their interaction with… read more here.
Keywords: cell receptor; antigen binding; phage display; interaction ... See more keywords
The antigen-binding fragment of human gamma immunoglobulin prevents amyloid β-peptide folding into β-sheet to form oligomers
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.17074
Abstract: The amyloid beta-peptide (Aβ) plays a leading role in Alzheimer's disease (AD) physiopathology. Even though monomeric forms of Aβ are harmless to cells, Aβ can aggregate into β-sheet oligomers and fibrils, which are both neurotoxic.… read more here.
Keywords: aggregation; antigen binding; human gamma; sheet ... See more keywords
Understanding the Significance and Implications of Antibody Numbering and Antigen-Binding Surface/Residue Definition
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2018.02278
Abstract: Monoclonal antibodies are playing an increasing role in both human and animal health. Different strategies of protein and chemical engineering, including humanization techniques of non-human antibodies were applied successfully to optimize clinical performances of antibodies.… read more here.
Keywords: definition; antigen binding; antibody; understanding significance ... See more keywords
Research progress on unique paratope structure, antigen binding modes, and systematic mutagenesis strategies of single-domain antibodies
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1059771
Abstract: Single-domain antibodies (sdAbs) showed the incredible advantages of small molecular weight, excellent affinity, specificity, and stability compared with traditional IgG antibodies, so their potential in binding hidden antigen epitopes and hazard detection in food, agricultural… read more here.
Keywords: unique paratope; antigen binding; paratope structure; antigen ... See more keywords