Antigen Retrieval for Immunostaining of Modified Cytosine Species.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_18
Abstract: Immunostaining (also called as immunofluorescence) is a fluorescence labeling method to stain one or more epitopes of interest on DNA and/or protein using specific antibodies. Cytosine modifications can be detected quantitatively by immunostaining. The protocol… read more here.
Keywords: immunostaining modified; antigen retrieval; retrieval immunostaining; antigen ... See more keywords
Antigen Retrieval and Signal Amplification.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_5
Abstract: Immunohistochemistry (IHC) offers a valuable method for determining the spatial distribution of proteins in cells and tissues. Fixation of tissues prior IHC enables their long-term stability and preserves tissue morphology; however, downstream analysis of protein… read more here.
Keywords: amplification; signal amplification; retrieval signal; antigen retrieval ... See more keywords
Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH
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DOI: 10.1038/s41596-020-00414-z
Abstract: Advances in light-sheet and confocal microscopy now allow imaging of cleared large biological tissue samples and enable the 3D appreciation of cell and protein localization in their native organ environment. However, the sample preparations for… read more here.
Keywords: whole organs; flash; antigen retrieval; microscopy ... See more keywords
Instant Pot for antigen retrieval: a simple, safe and economical method for use in immunohistochemistry.
Sign Up to like & getrecommendations! Published in 2023 at "BioTechniques"
DOI: 10.2144/btn-2022-0043
Abstract: Here, the authors report a simple method to perform antigen retrieval using a commonly available commercial Instant Pot® for immunohistochemistry. It provides a validated alternative to previous antigen retrieval methods that employ water baths, microwave… read more here.
Keywords: pot; immunohistochemistry; antigen retrieval; instant pot ... See more keywords