Modular Antigen‐Specific T‐cell Biofactories for Calibrated In Vivo Synthesis of Engineered Proteins
Sign Up to like & getrecommendations! Published in 2018 at "Advanced Biosystems"
DOI: 10.1002/adbi.201800210
Abstract: An artificial cell‐signaling pathway is developed that capitalizes on the T‐cell's innate extravasation ability and transforms it into a vector (T‐cell Biofactory) for synthesizing calibrated amounts of engineered proteins in vivo. The modularity of this… read more here.
Keywords: cell biofactory; specific cell; antigen specific; cell ... See more keywords
Tissue‐Targeted Drug Delivery Strategies to Promote Antigen‐Specific Immune Tolerance
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202202238
Abstract: During autoimmunity or organ transplant rejection, the immune system attacks host or transplanted tissue, causing debilitating inflammation for millions of patients. There is no cure for most of these diseases. Further, available therapies modulate inflammation… read more here.
Keywords: immune tolerance; tolerance; drug delivery; antigen specific ... See more keywords
Analysis of Antigen‐Specific Human Memory B Cell Populations Based on In Vitro Polyclonal Stimulation
Sign Up to like & getrecommendations! Published in 2020 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.109
Abstract: Antigen‐specific memory B cell (MBC) populations mediate the rapid, strong, and high‐affinity secondary antibody responses that play a key role in combating infection and generating protective responses to vaccination. Recently, cell staining with fluorochrome‐labeled antigens… read more here.
Keywords: stimulation; analysis; antigen specific; mbc ... See more keywords
Engineering antigen‐specific NK cell lines against the melanoma‐associated antigen tyrosinase via TCR gene transfer
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Immunology"
DOI: 10.1002/eji.201948140
Abstract: Introduction of Chimeric Antigen Receptors to NK cells has so far been the main practical method for targeting NK cells to specific surface antigens. In contrast, T cell receptor (TCR) gene delivery can supply large… read more here.
Keywords: cell; antigen; antigen specific; tcr gene ... See more keywords
Immunization with excretory‐secretory molecules of intestinal nematodes induces antigen‐specific protective memory Th2 cell responses
Sign Up to like & getrecommendations! Published in 2023 at "European Journal of Immunology"
DOI: 10.1002/eji.202250237
Abstract: Parasitic nematodes infect more than 1 billion people in the global south. The development of effective antihelminthic vaccines is a crucial tool for their future elimination. Protective immune responses to nematodes depend on Gata3+ Th2… read more here.
Keywords: immunization; infection; antigen specific; memory th2 ... See more keywords
Characterization and Activation of Fas Ligand-Producing Mouse B Cells and Their Killer Exosomes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1237-8_9
Abstract: B lymphocytes make several contributions to immune regulation including production of antibodies with regulatory properties, release of immune suppressive cytokines, and expression of death-inducing ligands. A role for Fas ligand (FasL)-expressing "killer" B cells in… read more here.
Keywords: characterization activation; killer; fas ligand; antigen specific ... See more keywords
CTL ELISPOT Assay and T Cell Detection.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_5
Abstract: Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector molecules, such as… read more here.
Keywords: elispot; elispot assay; antigen specific; ctl ... See more keywords
Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8938-6_3
Abstract: Human induced pluripotent stem cells (iPSCs) are a potential source of blood cells for transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. The development of widely varying reprogramming methods has enabled… read more here.
Keywords: human induced; antigen specific; specific cells; stem ... See more keywords
Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8958-4_6
Abstract: Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used… read more here.
Keywords: antibody secreting; monoclonal antibodies; specific antibody; antigen specific ... See more keywords
Detection of Antigen-Specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8976-8_31
Abstract: Hepatitis C virus-mediated immune suppression is an underlying feature leading to the establishment of viral persistence and chronic infection. In particular, HCV core protein has been shown to exhibit significant immunosuppressive activity of T cells… read more here.
Keywords: specific cd8; antigen specific; transgenic mice; core transgenic ... See more keywords
Development of Mold Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9591-2_8
Abstract: Common methods to quantify molds in the environment are based on the detection of viable and nonviable fungal components using cultivation technique or assessment by microscopy. These methods are time consuming and laborious and require… read more here.
Keywords: development mold; antigen specific; exposure; antigen ... See more keywords