Antisense Oligonucleotides Selectively Enter Human-derived Antibiotic-Resistant Bacteria through bacterial-specific ATP-binding Cassette Sugar Transporter.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced materials"
DOI: 10.1002/adma.202300477
Abstract: Current vehicles used to deliver antisense oligonucleotides (ASOs) cannot distinguish between bacterial and mammalian cells, greatly hindering the preclinical or clinical treatment of bacterial infections, especially those caused by antibiotic-resistant bacteria. Herein, we leverage the… read more here.
Keywords: atp binding; antisense oligonucleotides; binding cassette; antibiotic resistant ... See more keywords
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3350
Abstract: Ultrafiltration/diafiltration (UF/DF) has been the hallmark for concentrating and buffer exchange of protein and peptide-based therapeutics for years. Here we examine the capabilities and limitations of UF/DF membranes to process oligonucleotides using antisense oligonucleotides (ASOs)… read more here.
Keywords: diafiltration; use ultrafiltration; antisense oligonucleotides; processing antisense ... See more keywords
Evaluation of Gene Expression Knock‐Down by Chemically and Structurally Modified Gapmer Antisense Oligonucleotides
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200168
Abstract: We analyzed the effect of modified nucleotides within gapmer antisense oligonucleotides on RNase H mediated gene silencing. Additionally, short hairpins were introduced into antisense oligonucleotides as structural motifs, and their influence on biological and physicochemical… read more here.
Keywords: knock; antisense oligonucleotides; gene; gapmer antisense ... See more keywords
Modified U1 snRNA and antisense oligonucleotides rescue splice mutations in SLC26A4 that cause hereditary hearing loss
Sign Up to like & getrecommendations! Published in 2019 at "Human Mutation"
DOI: 10.1002/humu.23774
Abstract: One of most important factors for messenger RNA (mRNA) transcription is the spliceosomal component U1 small nuclear RNA (snRNA), which recognizes 5′ splicing donor sites at specific regions in pre‐mRNA. Mutations in these sites disrupt… read more here.
Keywords: modified snrna; antisense oligonucleotides; hearing loss; snrna antisense ... See more keywords
Development and Clinical Applications of Antisense Oligonucleotide Gapmers.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0771-8_2
Abstract: DNA-like molecules called antisense oligonucleotides have opened new treatment possibilities for genetic diseases by offering a method of regulating gene expression. Antisense oligonucleotides are often used to suppress the expression of mutated genes which may… read more here.
Keywords: treatment; chemistry; antisense oligonucleotide; antisense oligonucleotides ... See more keywords
In Vivo Administration of Therapeutic Antisense Oligonucleotides.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1158-6_17
Abstract: With the rapid revolution in RNA/DNA sequencing technologies, it is evident that mammalian genomes express tens of thousands of long noncoding RNAs (lncRNAs). Since a large majority of lncRNAs have been functionally implicated in cancer… read more here.
Keywords: administration; vivo administration; therapeutic antisense; chemistry ... See more keywords
Design and In Vitro Use of Antisense Oligonucleotides to Correct Pre-mRNA Splicing Defects in Inherited Retinal Dystrophies.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7522-8_5
Abstract: Antisense oligonucleotides (AONs) are small molecules able to bind to the pre-mRNA and modulate splicing. The increasing amount of intronic mutations leading to pseudoexon insertion in genes underlying inherited retinal dystrophies (IRDs) has highlighted the… read more here.
Keywords: correct pre; retinal dystrophies; mrna splicing; pre mrna ... See more keywords
Specific Increase of Protein Levels by Enhancing Translation Using Antisense Oligonucleotides Targeting Upstream Open Frames.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-10-4310-9_9
Abstract: A number of diseases are caused by low levels of key proteins; therefore, increasing the amount of specific proteins in human bodies is of therapeutic interest. Protein expression is downregulated by some structural or sequence… read more here.
Keywords: protein; increase; translation; protein levels ... See more keywords
Sarm1 haploinsufficiency or low expression levels after antisense oligonucleotides delay programmed axon degeneration
Sign Up to like & getrecommendations! Published in 2021 at "Cell Reports"
DOI: 10.1016/j.celrep.2021.110108
Abstract: Summary Activation of the pro-degenerative protein SARM1 after diverse physical and disease-relevant injuries causes programmed axon degeneration. Original studies indicate that substantially decreased SARM1 levels are required for neuroprotection. However, we demonstrate, in Sarm1 haploinsufficient… read more here.
Keywords: programmed axon; degeneration; axon degeneration; sarm1 levels ... See more keywords
Targeting nanocarriers containing antisense oligonucleotides to cancer cell
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Drug Delivery Science and Technology"
DOI: 10.1016/j.jddst.2016.12.001
Abstract: Abstract Treatment of cancer is a furtive problem in the current era in spite of significant advancement in drug delivery systems and identification of new therapeutic molecules. The ration of lab to market translation of… read more here.
Keywords: containing antisense; cancer; targeting nanocarriers; cell ... See more keywords
Cationic Micelles Outperform Linear Polymers for Delivery of Antisense Oligonucleotides in Serum: An Exploration of Polymer Architecture, Cationic Moieties, and Cell Addition Order.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.2c00379
Abstract: Antisense oligonucleotides (ASOs) are an important emerging therapeutic; however, they struggle to enter cells without a delivery vehicle, such as a cationic polymer. To understand the role of polymer architecture for ASO delivery, five linear… read more here.
Keywords: polymer; polymer architecture; antisense oligonucleotides; delivery ... See more keywords