Development of aptamer‐based ELISA method for d‐dimer detection
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2347
Abstract: This study developed an aptamer‐based enzyme‐linked immunosorbent assay (ELISA) system for sensitive detection of the d ‐dimer molecule, which is significantly elevated in several severe diseases. Aptamers are known to have advantages, such as longer… read more here.
Keywords: system; aptamer based; dimer; detection ... See more keywords
An aptamer‐based paper microfluidic device for the colorimetric determination of cocaine
Sign Up to like & getrecommendations! Published in 2018 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700254
Abstract: A method utilizing paper microfluidics coupled with gold nanoparticles and two anticocaine aptamers has been developed to detect seized cocaine samples. The ready‐to‐use format involves the use of a paper strip that produces a color… read more here.
Keywords: based paper; paper microfluidic; aptamer based; paper ... See more keywords
Aptamer‐Based Cancer Cell Analysis and Treatment
Sign Up to like & getrecommendations! Published in 2022 at "ChemistryOpen"
DOI: 10.1002/open.202200141
Abstract: Abstract Aptamers are a class of single‐stranded DNA or RNA oligonucleotides that can exclusively bind to various targets with high affinity and selectivity. Regarded as “chemical antibodies”, aptamers possess several intrinsic advantages, including easy synthesis,… read more here.
Keywords: analysis treatment; cancer; based cancer; aptamer based ... See more keywords
Impedimetric Aptamer-Based Biosensors: Principles and Techniques.
Sign Up to like & getrecommendations! Published in 2020 at "Advances in biochemical engineering/biotechnology"
DOI: 10.1007/10_2019_113
Abstract: Aptamers are a specific class of ligands with high affinities comparable to antibodies, which are selected and synthesized in vitro. In combination with impedance spectroscopy as sensitive measurement method, we gain a class of biosensors… read more here.
Keywords: aptamer based; based biosensors; biosensors principles; impedimetric aptamer ... See more keywords
Application of an Aptamer-Based Proteomics Assay (SOMAscan™) in Rat Cerebrospinal Fluid.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9706-0_13
Abstract: The exploration of the cerebrospinal fluid (CSF) through proteomics techniques might help in the search of molecular biomarkers relevant to neurological pathologies. Aiming this, we describe here a commercially available multiplexed proteomics technology based on… read more here.
Keywords: aptamer based; based proteomics; application aptamer; rat ... See more keywords
Aptamer-based fluorescence-quenching lateral flow strip for rapid detection of mercury (II) ion in water samples
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0491-7
Abstract: AbstractDivalent mercury ion (Hg2+) is one of the most common and stable forms of mercury pollution. In this study, a skillfully designed lateral flow strip (LFS) was developed for sensitive detection of Hg2+ in river… read more here.
Keywords: fluorescence quenching; detection; aptamer based; based fluorescence ... See more keywords
Microfluidic flow-injection aptamer-based chemiluminescence platform for sulfadimethoxine detection
Sign Up to like & getrecommendations! Published in 2022 at "Mikrochimica Acta"
DOI: 10.1007/s00604-022-05216-6
Abstract: Gold nanoparticle–catalyzed chemiluminescence (CL) of luminol is an attractive alternative to strategies relying on enzymes, as their aggregation leads to significantly enhanced CL signals. Consequently, analytes disturbing such aggregation will lead to an easy-to-quantify weakening… read more here.
Keywords: aptamer based; flow injection; detection; platform ... See more keywords
Modifications of the chromophore of Spinach aptamer based on QM:MM calculations
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Molecular Modeling"
DOI: 10.1007/s00894-017-3232-0
Abstract: Spinach aptamer was developed as an RNA analog of the green fluorescent protein. The aptamer interacts with its ligand and modifies its electronic spectrum so that it fluoresces brightly at the wavelength of 501 nm. Song… read more here.
Keywords: chromophore spinach; spinach aptamer; aptamer based; modifications chromophore ... See more keywords
Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-02641-7
Abstract: ObjectiveWe developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication initiator protein derived from porcine circovirus type 2 (pRep).ResultsFor construction of DNA aptamer and NanoLuc conjugate… read more here.
Keywords: sandwich assay; based sandwich; aptamer based; dna nanoluc ... See more keywords
Engineering an aptamer-based recognition sensor for electrochemical opium alkaloid biosensing
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Materials Science: Materials in Electronics"
DOI: 10.1007/s10854-018-00618-w
Abstract: Here we propose an electrochemical aptamer-based sensing strategy for sensitive detection of the codeine (COD). Platform construction was started by decoration NH2-functionalized Fe3O4 with gold nanoparticles (Fe3O4/AuNPs). Carbon nanotubes were then placed on a glassy… read more here.
Keywords: detection; aptamer based; surface; cod ... See more keywords
Aptamer-Based Detection Methodology Studies in Food Safety
Sign Up to like & getrecommendations! Published in 2019 at "Food Analytical Methods"
DOI: 10.1007/s12161-019-01437-3
Abstract: Oligonucleotide aptamers as a new type of molecular recognition element, compared with conventional antibodies, have not only high affinity and specificity to target molecules but also many advantages such as a wide range of target… read more here.
Keywords: methodology; food safety; aptamer based; methodology studies ... See more keywords