Determination of Aqueous Two‐Phase System Binodals and Tie‐Lines by Electrowetting‐on‐Dielectric Droplet Manipulation
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800553
Abstract: Handling the aqueous two‐phase systems (ATPSs) formed by liquid–liquid phase separation (LLPS) relies on the accurate construction of binodal curves and tie‐lines, which delineate the polymer concentrations required for phase separation and depict the properties… read more here.
Keywords: tie lines; phase; droplet manipulation; aqueous two ... See more keywords
Purification of bacterial inulinase in aqueous two‐phase systems
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DOI: 10.1002/elsc.201700174
Abstract: In this study, extracellular inulinase from Bacillus sp. 11/3 was partially purified and concentrated using aqueous two‐phase system (ATPS). Two different phase forming salts and four types of polyethylene glycol (PEG) were used. Binodal curves… read more here.
Keywords: phase; two phase; inulinase; inulinase purification ... See more keywords
Cell-laden microgel prepared using a biocompatible aqueous two-phase strategy
Sign Up to like & getrecommendations! Published in 2017 at "Biomedical Microdevices"
DOI: 10.1007/s10544-017-0198-8
Abstract: Microfluidic methods are frequently used to produce cell-laden microgels for various biomedical purposes. Such microfluidic methods generally employ oil-water systems. The poor distribution of crosslinking reagents in the oil phase limits the available gelation strategies.… read more here.
Keywords: phase; cell laden; microgel; two phase ... See more keywords
Modification of the Extended UNIQUAC Model to Study the Phase Behavior of the [Bmim]Cl–K2HPO4 Aqueous Two Phase System at 298.15 K
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Solution Chemistry"
DOI: 10.1007/s10953-017-0622-y
Abstract: Aqueous two phase systems (ATPS), consisting of two incompatible aqueous solutions, are widely used in biological extraction. In recent years, many experimental studies on the phase behavior of ionic liquid(IL)–inorganic salt ATPS systems have been… read more here.
Keywords: extended uniquac; phase; two phase; model ... See more keywords
Extraction and purification of Polyhydroxyalkanoates (PHAs): application of Thermoseparating aqueous two-phase extraction
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DOI: 10.1007/s10965-017-1307-3
Abstract: Being biodegradable, non-toxic and renewable as well as having similar or better properties than commercial plastics, polyhydroxyalkanoates (PHAs) can be a potential game changer in the polymer industry. Although viewed as a sustainable alternative to… read more here.
Keywords: phase; extraction; two phase; polyhydroxyalkanoates phas ... See more keywords
Simultaneous extraction of antibiotic and estrogen from animal blood serum using aqueous two-phase systems as predictor of environmental impact
Sign Up to like & getrecommendations! Published in 2020 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-020-07770-z
Abstract: Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid… read more here.
Keywords: phase; extraction; two phase; blood serum ... See more keywords
Response surface methodology for the evaluation of guanidine hydrochloride partitioning in polymer-salt aqueous two-phase system
Sign Up to like & getrecommendations! Published in 2017 at "Korean Journal of Chemical Engineering"
DOI: 10.1007/s11814-017-0108-z
Abstract: The current study employed response surface methodology (RSM) with a face-centered central composite design (CCD) to indicate the essential variables on the partition coefficient of guanidine hydrochloride (GuHCl) in the poly (ethylene glycol) (PEG)-phosphate aqueous… read more here.
Keywords: guanidine hydrochloride; methodology; two phase; response surface ... See more keywords
Partition Behaviors of Different Polar Anthocyanins in Aqueous Two-Phase Systems and Extraction of Anthocyanins from Nitraria tangutorun Bobr. and Lycium ruthenicum Murr.
Sign Up to like & getrecommendations! Published in 2017 at "Food Analytical Methods"
DOI: 10.1007/s12161-017-1071-3
Abstract: This study aimed to investigate the partition behaviors of various polar anthocyanins in NaH2PO4/(NH4)2SO4-ethanol aqueous two-phase systems (ATPS) and to extract anthocyanins from Nitraria tangutorun Bobr. and Lycium ruthenicum Murr. Anthocyanins in Hibiscus sabdariffa L.,… read more here.
Keywords: phase; two phase; partition behaviors; ruthenicum ... See more keywords
Green Choline Amino Acid Ionic Liquid Aqueous Two-Phase Extraction Coupled with HPLC for Analysis Sunset Yellow in Beverages
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DOI: 10.1007/s12161-019-01603-7
Abstract: An analytical method based on amino acid ionic liquid aqueous two-phase extraction (ATPE) coupled with HPLC-UV detection has been developed for the determination of sunset yellow in beverages. Experimental parameters of ATPE including ionic liquids,… read more here.
Keywords: sunset yellow; phase; ionic liquid; two phase ... See more keywords
Biotechnological purification of a β-fructofuranosidase (β-FFase) from Aspergillus tamarii kita: Aqueous two-phase system (PEG/Citrate) and biochemical characterization
Sign Up to like & getrecommendations! Published in 2021 at "Biocatalysis and Agricultural Biotechnology"
DOI: 10.1016/j.bcab.2021.102070
Abstract: Abstract β-fructofuranosidases (EC3.2.1.26) are members of the GH32 family of glycoside hydrolases, which include more than 390 enzymes of vegetable and microbial origins, used in several biotechnological applications. Thus, this research aimed to produce a… read more here.
Keywords: phase; fructofuranosidase; citrate; aspergillus tamarii ... See more keywords
An efficient aqueous two phase systems using dual inorganic electrolytes to separate 1,3-propanediol from the fermented broth.
Sign Up to like & getrecommendations! Published in 2018 at "Bioresource technology"
DOI: 10.1016/j.biortech.2018.01.076
Abstract: An aqueous two phase extraction using K2CO3:K2HPO4/Isoproponal was investigated for the recovery of 1,3-propanediol from the fermented broth. Initially, the concentration of K2CO3 on phase formation, the partition co-efficient and recovery of 1,3-PDO was evaluated… read more here.
Keywords: phase; fermented broth; propanediol fermented; two phase ... See more keywords