Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6563-2_21
Abstract: Argonaute proteins are key components of the microRNA-induced silencing complexes (miRISCs) that mediate the posttranscriptional gene silencing of microRNAs and small interfering RNA (siRNAs). The complex reaction mechanism of miRISC is expected to be characterized… read more here.
Keywords: argonaute; molecule fluorescence; single molecule; reaction pathways ... See more keywords
Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7165-7_9
Abstract: Argonaute (AGO) proteins play a key role in RNA silencing mechanisms. RNA silencing affects both RNA degradation and translation. The characterization of translation-associated RNA silencing mechanisms and components often requires polysome isolation and analysis. In… read more here.
Keywords: association polysomes; detection argonaute; argonaute; rna silencing ... See more keywords
Argonaute Target Search is Facilitated by Long Distance Diffusion
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1701
Abstract: Argonaute proteins have a variety of functions ranging from post transcriptional gene silencing in eukaryotes to host defence in prokaryotic systems. Despite the well-established functional understanding of Argonautes, the biophysical understanding of its targeting mechanism… read more here.
Keywords: target search; argonaute; diffusion; long distance ... See more keywords
Argonaute Facilitates the Lateral Diffusion of the Guide along Its Target and Prevents the Guide from Being Pushed Away by the Ribosome.
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DOI: 10.1021/acs.biochem.8b00213
Abstract: Argonaute (AGO) proteins play central roles in nucleic acid-guided interference that regulates gene expression and defend against foreign genetic elements in all life. Although much progress has been made with respect to the function of… read more here.
Keywords: along target; argonaute; diffusion; target ... See more keywords
The dsRBP Staufen2 governs RNP assembly of neuronal Argonaute proteins
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac487
Abstract: Abstract Mature microRNAs are bound by a member of the Argonaute (Ago1-4) protein family, forming the core of the RNA-induced silencing complex (RISC). Association of RISC with target mRNAs results in ribonucleoprotein (RNP) assembly involved… read more here.
Keywords: governs rnp; rnp; argonaute; staufen2 governs ... See more keywords
The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz040
Abstract: Abstract Argonaute proteins are present and conserved in all domains of life. Recently characterized prokaryotic Argonaute proteins (pAgos) participates in host defense by DNA interference. Here, we report that the Natronobacterium gregoryi Argonaute (NgAgo) enhances… read more here.
Keywords: proteins enhance; prokaryotic argonaute; argonaute; enhance homology ... See more keywords
Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/gr.276578.122
Abstract: Argonaute 2 (AGO2) is a ubiquitously expressed protein critical for regulation of mRNA translation and vital to animal development. AGO2 protein is found in both cytoplasmic and nuclear compartments, and although its cytoplasmic role is… read more here.
Keywords: protein; argonaute; mammalian male; male reproduction ... See more keywords
Prokaryotic Argonaute Protein from Natronobacterium gregoryi Requires RNAs To Activate for DNA Interference In Vivo
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DOI: 10.1128/mbio.03656-21
Abstract: We revealed the mechanism that explains how the NgAgo eliminates the invading foreign DNA and bacteriophage in bacterial cells at 37°C, and by leveraging this discovery, NgAgo can be programmed to target a plasmid or… read more here.
Keywords: natronobacterium gregoryi; argonaute; mechanism; bacteriophage ... See more keywords
Genome-Wide Identification of the Argonaute Protein Family and Its Expression Analysis under PEG6000, ABA and Heat Treatments in Populus alba × P. glandulosa
Sign Up to like & getrecommendations! Published in 2023 at "Forests"
DOI: 10.3390/f14051015
Abstract: The argonaute (AGO) protein, as an important member of the small RNA (sRNA) regulatory pathway gene-silencing complex (RNA-induced silencing complex, RISC), is a key protein that mediates gene silencing and plays a key role in… read more here.
Keywords: family; ptago genes; argonaute; expression analysis ... See more keywords