Articles with "arms pcr" as a keyword



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A novel one-step amplification refractory mutation system PCR (ARMS-PCR) for differentiation of canine parvovirus-2 variants.

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Published in 2021 at "Virus genes"

DOI: 10.1007/s11262-021-01861-w

Abstract: Enteritis caused by CPV-2 antigenic variants (CPV-2a, 2b, and 2c) is frequently reported in dogs worldwide leading to significant morbidity and mortality. Here, we describe about a simple, single-step, ARMS-PCR strategy targeting the mutant 426… read more here.

Keywords: cpv; typed cpv; arms pcr; cpv antigenic ... See more keywords
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Multiplex DNA methylation profiling by ARMS-PCR for body fluid identification

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Published in 2019 at "Forensic Science International: Genetics Supplement Series"

DOI: 10.1016/j.fsigss.2019.10.189

Abstract: Abstract A set of CpG sites were detected and evaluated to identify body fluids based on DNA methylation using the amplification refractory mutation system-PCR (ARMS-PCR). In this research, two multiplex DNA methylation reactions composed of… read more here.

Keywords: methylation; arms pcr; methylation profiling; dna methylation ... See more keywords
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Visual Detection of ACE I/D Polymorphism Using T-ARMS-PCR Combined with a Lateral Flow Assay and Its Clinical Application.

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Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.1c04817

Abstract: Insertions/deletions (indels) variations have been recognized as a promising marker for the development of various diseases. However, methods used for the genotyping of indels in studies were tedious, complicated, and required sophisticated or expensive instruments,… read more here.

Keywords: lateral flow; flow assay; ace; arms pcr ... See more keywords
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Sensitive Detection of a Single-Nucleotide Polymorphism in Foodborne Pathogens Using CRISPR/Cas12a-Signaling ARMS-PCR.

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Published in 2022 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.2c03304

Abstract: Salmonella infection, particularly that caused by drug-resistant strain, has become a worldwide public health issue. Herein, we presented a CRISPR/Cas12a-signaling ARMS-PCR assay, termed cARMS, capable of sensitively detecting drug-resistant Salmonella enterica (S. enterica) involving single-nucleotide… read more here.

Keywords: nucleotide polymorphism; single nucleotide; arms pcr; cas12a signaling ... See more keywords
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Comment on: BRAF mutation analysis by ARMS‐PCR refines thyroid nodule management

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Published in 2019 at "Clinical Endocrinology"

DOI: 10.1111/cen.14103

Abstract: we read the paper by Li and colleagues1 , aiming to explore the clinical significance of the BRAF p.V600E mutation in a preoperative setting, as an aid to decision-making. In order to improve the pre-operative… read more here.

Keywords: arms pcr; analysis; comment braf; braf mutation ... See more keywords
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Comprehensive analysis of EGFR T790M detection by ddPCR and ARMS-PCR and the effect of mutant abundance on the efficacy of osimertinib in NSCLC patients.

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Published in 2019 at "Journal of thoracic disease"

DOI: 10.21037/jtd.2019.07.42

Abstract: Background Patients with non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutations often develop systemic disease progression during treatment with EGFR-tyrosine kinase inhibitors (TKIs). Droplet digital polymerase chain reaction (ddPCR) and amplification… read more here.

Keywords: ddpcr arms; abundance; arms pcr; t790m ... See more keywords

Characterization of the genetic polymorphism linked to the β-casein A1/A2 alleles using different molecular and biochemical methods.

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Published in 2022 at "Journal of dairy science"

DOI: 10.3168/jds.2022-22136

Abstract: The 2 major subvariants of β-casein (A1 and A2), coded by CSN2 gene, have received great interest in the last decade both from the scientific community and the dairy sector due to their influence on… read more here.

Keywords: casein; large scale; arms pcr; pcr ... See more keywords
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Diagnostic Accuracy of Droplet Digital PCR and Amplification Refractory Mutation System PCR for Detecting EGFR Mutation in Cell-Free DNA of Lung Cancer: A Meta-Analysis

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Published in 2020 at "Frontiers in Oncology"

DOI: 10.3389/fonc.2020.00290

Abstract: Background: Epidermal growth factor receptor (EGFR) mutation testing in plasma cell-free DNA (cfDNA) from advanced lung cancer patients is an emerging clinical tool. This meta-analysis was designed to determine the diagnostic accuracy of two common… read more here.

Keywords: egfr mutation; arms pcr; detecting egfr; sensitivity ... See more keywords
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Molecular Determination of Vascular Endothelial Growth Factor, miRNA-423 Gene Abnormalities by Utilizing ARMS-PCR and Their Association with Fetal Hemoglobin Expression in the Patients with Sickle Cell Disease

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Published in 2022 at "Current Issues in Molecular Biology"

DOI: 10.3390/cimb44060175

Abstract: Recent studies have indicated that microRNA and VEGF are considered to be genetic modifiers and are associated with elevated levels of fetal haemoglobin HbF, and thus they reduce the clinical impact of sickle haemoglobin (HbS)… read more here.

Keywords: mir 423; 423 genotype; genotype; arms pcr ... See more keywords
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Design, Optimization and Validation of the ARMS PCR Protocol for the Rapid Diagnosis of Wilson’s Disease Using a Panel of 14 Common Mutations for the European Population

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Published in 2022 at "Genes"

DOI: 10.3390/genes13111940

Abstract: Background: Wilson’s disease (WD) is an autosomal recessive inherited disorder of copper metabolism resulting from various mutations in the ATP7B gene. Despite good knowledge and successful treatment options, WD is a severe disease that leads… read more here.

Keywords: common mutations; disease; diagnosis; wilson disease ... See more keywords