Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-13532-z
Abstract: High-efficiency gene editing in primary human cells is critical for advancing therapeutic development and functional genomics, yet conventional electroporation platforms often require high cell input and are poorly suited to parallelized experiments. Here we introduce… read more here.
Keywords: screening primary; primary human; arrayed crispr; human cells ... See more keywords
A Whole Genome-Wide Arrayed CRISPR Screen in Primary Organ Fibroblasts to Identify Regulators of Kidney Fibrosis
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DOI: 10.1177/2472555220915851
Abstract: Kidney fibrosis presents a hallmark of chronic kidney disease. With ever-increasing patient numbers and limited treatment options available, novel strategies for therapeutic intervention in kidney disease are warranted. Fibrosis commonly results from a wound healing… read more here.
Keywords: genome wide; fibrosis; whole genome; kidney fibrosis ... See more keywords
A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments
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DOI: 10.1371/journal.pone.0307445
Abstract: An arrayed CRISPR screen is a high-throughput functional genomic screening method, which typically uses 384 well plates and has different gene knockouts in different wells. Despite various computational workflows, there is currently no systematic way… read more here.
Keywords: arrayed crispr; simulation model; crispr screen; statistical simulation ... See more keywords