Characterization and use of Tetrahymena thermophila artificial chromosome 2 (TtAC2) constructed by biomimetic of macronuclear rDNA minichromosome.
Sign Up to like & getrecommendations! Published in 2021 at "Microbiological research"
DOI: 10.1016/j.micres.2021.126764
Abstract: Efficient expression vectors for unicellular ciliate eukaryotic Tetrahymena thermophila are still needed in recombinant biology and biotechnology applications. Previously, the construction of the T. thermophila Macronuclear Artificial Chromosome 1 (TtAC1) vector revealed additional needs for… read more here.
Keywords: ttac2; tetrahymena thermophila; rdna; artificial chromosome ... See more keywords
Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons
Sign Up to like & getrecommendations! Published in 2020 at "Experimental Cell Research"
DOI: 10.1016/j.yexcr.2020.111840
Abstract: Gene expression studies and gene therapy require efficient gene delivery into cells. Different technologies by viral and non-viral mechanisms have been used for gene delivery into cells. Small gene vectors transfer across the cell membrane… read more here.
Keywords: vector; delivery; human artificial; artificial chromosome ... See more keywords
Purification of Yeast Artificial Chromosome DNA for Microinjection Using a Two-Gel Electrophoresis Procedure.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot093930
Abstract: This protocol describes a method to purify yeast artificial chromosome (YAC) DNA for microinjection. YAC DNA solutions cannot be concentrated by standard DNA precipitation methods and resuspended into smaller volumes. Attempts to precipitate YAC DNA… read more here.
Keywords: yeast artificial; gel electrophoresis; dna; yac dna ... See more keywords
One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of Plasmids.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot098095
Abstract: In the one-step approach to bacterial artificial chromosome (BAC) modification, two plasmids are introduced into the BAC host cells. The shuttle pLD53.SC2, carrying the EFGP reporter sequence and requiring the π protein to replicate, must… read more here.
Keywords: bac; bac modification; one step; chromosome bac ... See more keywords