Ferriporphyrin-inspired MOFs as an artificial metalloenzyme for highly sensitive detection of H2O2 and glucose
Sign Up to like & getrecommendations! Published in 2020 at "Chinese Chemical Letters"
DOI: 10.1016/j.cclet.2020.03.052
Abstract: Abstract Metalloenzymes which employ metal species and organic ligands as central active sites play significant roles in various biological activities. Development of artificial metalloenzymes can help to understand the related physiological mechanism and promote the… read more here.
Keywords: h2o2 glucose; ferriporphyrin inspired; artificial metalloenzyme; highly sensitive ... See more keywords
Electrochemical characterization of the artificial metalloenzyme papain-[(η6-arene)Ru(1,10-phenanthroline)Cl]+
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Electroanalytical Chemistry"
DOI: 10.1016/j.jelechem.2020.113882
Abstract: Abstract Electrochemical properties were studied for [(η6-arene)Ru(1,10-phenanthroline)Cl]Cl (arene = C6H5(CH2)2NHCOCH2Cl) organometallic complex 1, protein Papain PAP and its conjugate with organometallic complex 1-PAP. The latter can serve as an artificial metalloenzyme with catalytic activity in transfer hydrogenation.… read more here.
Keywords: electrochemical characterization; artificial metalloenzyme; arene phenanthroline; papain ... See more keywords
Chaperonin-Nanocaged Hemin as an Artificial Metalloenzyme for Oxidation Catalysis.
Sign Up to like & getrecommendations! Published in 2017 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.7b08963
Abstract: Taking inspiration from biology's effectiveness in functionalizing protein-based nanocages for chemical processes, we describe here a rational design of an artificial metalloenzyme for oxidations with the bacterial chaperonin GroEL, a nanocage for protein folding in… read more here.
Keywords: hemin groel; artificial metalloenzyme; hemin; oxidation ... See more keywords
Cofactor Binding Dynamics Influence the Catalytic Activity and Selectivity of an Artificial Metalloenzyme
Sign Up to like & getrecommendations! Published in 2020 at "ACS Catalysis"
DOI: 10.1021/acscatal.0c01619
Abstract: We present an artificial metalloenzyme based on the transcriptional regulator LmrR that exhibits dynamics involving the positioning of its abiological metal cofactor. The position of the cofactor, in turn, was found to be related to… read more here.
Keywords: cofactor; activity selectivity; artificial metalloenzyme;
Chemogenetic Evolution of a Peroxidase-like Artificial Metalloenzyme
Sign Up to like & getrecommendations! Published in 2021 at "ACS Catalysis"
DOI: 10.1021/acscatal.1c00134
Abstract: Directed evolution has helped enzyme engineering to remarkable successes in the past. A main challenge in directed evolution is to find the most suitable starting point, that is, an enzyme that all... read more here.
Keywords: like artificial; peroxidase like; evolution peroxidase; chemogenetic evolution ... See more keywords
An Artificial Metalloenzyme Based on a Copper Heteroscorpionate Enables sp3 C–H Functionalization via Intramolecular Carbene Insertion
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03311
Abstract: The selective functionalization of sp3 C–H bonds is a versatile tool for the diversification of organic compounds. Combining attractive features of homogeneous and enzymatic catalysts, artificial metalloenzymes offer an ideal means to selectively modify these… read more here.
Keywords: functionalization; copper heteroscorpionate; artificial metalloenzyme; metalloenzyme ... See more keywords
Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Barrel Protein Scaffold
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.3c00581
Abstract: Evolutionary engineering of our previously reported Cp*Rh(III)-linked artificial metalloenzyme was performed based on a DNA recombination strategy to improve its catalytic activity toward C(sp2)–H bond functionalization. Improved scaffold design was achieved with α-helical cap domains… read more here.
Keywords: evolutionary engineering; artificial metalloenzyme; metalloenzyme; iii ... See more keywords
On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme
Sign Up to like & getrecommendations! Published in 2018 at "Communications Chemistry"
DOI: 10.1038/s42004-018-0087-y
Abstract: Metal-catalyzed chemical transformations performed at the cellular level bear great potential for the manipulation of biological processes. The complexity of the cell renders the use of transition metal chemistry difficult in cellular systems. The delivery… read more here.
Keywords: artificial metalloenzyme; surface; chemistry; surface engineering ... See more keywords
A metal ion regulated artificial metalloenzyme.
Sign Up to like & getrecommendations! Published in 2017 at "Dalton transactions"
DOI: 10.1039/c7dt00533d
Abstract: Regulation of enzyme activity is essential in living cells. The rapidly increasing number of designer enzymes with new-to-nature activities makes it necessary to develop novel strategies for controlling their catalytic activity. Here we present the… read more here.
Keywords: artificial metalloenzyme; metal ion; ion regulated; metalloenzyme ... See more keywords
Towards Cellular Activation of an Artificial Metalloenzyme: Development of an Artificial Zymogen.
Sign Up to like & getrecommendations! Published in 2017 at "Chimia"
DOI: 10.2533/chimia.2017.199
Abstract: Regulation of enzymatic activity is essential for living organisms. Nowadays, with the emergence of synthetic biology, there is a rising interest in placing the activity of synthetic catalysts under the control of a cell. This… read more here.
Keywords: artificial zymogen; artificial metalloenzyme; development artificial; towards cellular ... See more keywords