Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7315-6_12
Abstract: Besides genome editing, the CRISPR-Cas9-based platform provides a new way of engineering artificial transcription factors (ATFs). Multiplex of guide RNA (gRNA) expression cassettes holds a great promise for many useful applications of CRISPR-Cas9. In this… read more here.
Keywords: transcription factors; artificial transcription; crispr; crispr dcas9 ... See more keywords
COMPASS for rapid combinatorial optimization of biochemical pathways based on artificial transcription factors
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DOI: 10.1038/s41467-019-10224-x
Abstract: Balanced expression of multiple genes is central for establishing new biosynthetic pathways or multiprotein cellular complexes. Methods for efficient combinatorial assembly of regulatory sequences (promoters) and protein coding sequences are therefore highly wanted. Here, we… read more here.
Keywords: artificial transcription; compass rapid; expression; transcription factors ... See more keywords
Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2021.676900
Abstract: The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales. Despite the fundamental role that artificial transcription factors (ATFs)… read more here.
Keywords: artificial transcription; plant; pichia pastoris; expression ... See more keywords