Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage
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DOI: 10.1039/d2sc05463a
Abstract: Cas12a is one of the most commonly used Cas proteins for genome editing and gene regulation. The first key step for Cas12a to fulfill its function is to search for its target among numerous nonspecific… read more here.
Keywords: ascas12a; diffusion; search; target search ... See more keywords